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WDR46 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR46 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR46 antibodies →
$504.00each
Catalog Number: LS-G128227-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR46 (NM_001164267), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WD repeat domain 46 | BING4 | UTP7 | C6orf11 | FP221 | WDR46
Origin Species
Human
NCBI GenBank
NM_005452

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.6 kda
Protein Type
Lysate
Amino Acid Sequence
1METAPKPGKD VPPKKDKLQT KRKKPREWKN PESQRGLSGT QDPFPGPAPV PVEVVQKFCR
61IDKSRKLPHS KAKTRSRLEV AEAEEEETSI KAARSELLLA EEPGFLEGED GEDTAKICQA
121DIVEAVDIAS AAKHFDLNLR QFGPYRLNYS RTGRHLAFGG RRGHVAALDW VTKKLMCEIN
181VMEAVRDIRF LHSEALLAVA QNRWLHIYDN QGIELHCIRR CDRVTRLEFL PFHFLLATAS
241ETGFLTYLDV SVGKIVAALN ARAGRLDVMS QNPYNAVIHL GHSNGTVSLW SPAMKEPLAK
301ILCHRGGVRA VAVDSTGTYM ATSGLDHQLK IFDLRGTYQP LSTRTLPHGA GHLAFSQRGL
361LVAGMGDVVN IWAGQGKASP PSLEQPYLTH RLSGPVHGLQ FCPFEDVLGV GHTGGITSML
421VPGAGEPNFD GLESNPYRSR KQRQEWEVKA LLEKVPAELI CLDPRALAEV DVISLEQGKK
481EQIERLGYDP QAKAPFQPKP KQKGRSSTAS LVKRKRKVMD EEHRDKVRQS LQQQHHKEAK
541AKPTGARPSA LDRFVR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

556 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.