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WDR43 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR43 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR43 antibodies →
$725.00each
Catalog Number: LS-G127082-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR43 (NM_015131), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0007 | WDR43 | WD repeat domain 43 | NET12 | UTP5
Origin Species
Human
NCBI GenBank
D26488
SwissProt
Q15061

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGGGGSCD PLAPAGVPCA FSPHSQAYFA LASTDGHLRV WETANNRLHQ EYVPSAHLSG
61TCTCLAWAPA RLQAKESPQR KKRKSEAVGM SNQTDLLALG TAVGSILLYS TVKGELHSKL
121ISGGHDNRVN CIQWHQDSGC LYSCSDDKHI VEWNVQTCKV KCKWKGDNSS VSSLCISPDG
181KMLLSAGRTI KLWVLETKEV YRHFTGHATP VSSLMFTTIR PPNESQPFDG ITGLYFLSGA
241VHDRLLNVWQ VRSENKEKSA VMSFTVTDEP VYIDLTLSEN KEEPVKLAVV CRDGQVHLFE
301HILNGYCKKP LTSNCTIQIA TPGKGKKSTP KPIPILAAGF CSDKMSLLLV YGSWFQPTIE
361RVALNSREPH MCLVRDISNC WAPKVETAIT KVRTPVMNSE AKVLVPGIPG HHAAIKPAPP
421QTEQVESKRK SGGNEVSIEE RLGAMDIDTH KKGKEDLQTN SFPVLLTQGL ESNDFEMLNK
481VLQTRNVNLI KKTVLRMPLH TIIPLLQELT KRLQGHPNSA VLMVQWLKCV LTVHASYLST
541LPDLVPQLGT LYQLMESRVK TFQKLSHLHG KLILLITQVT ASEKTKGATS PGQKAKLVYE
601EESSEEESDD EIADKDSEDN WDEDEEESES EKDEDVEEED EDAEGKDEEN GEDRDTASEK
661ELNGDSDLDP ENESEEE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

677 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.