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WDR37 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR37 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR37 antibodies →
$725.00each
Catalog Number: LS-G114191-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR37 (NM_014023), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0982 | RP11-529L18.2 | WD repeat domain 37 | WDR37
Origin Species
Human
NCBI GenBank
NM_014023
SwissProt
Q9Y2I8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
54.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPTESASCST ARQTKQKRKS HSLSIRRTNS SEQERTGLPR DMLEGQDSKL PSSVRSTLLE
61LFGQIEREFE NLYIENLELR REIDTLNERL AAEGQAIDGA ELSKGQLKTK ASHSTSQLSQ
121KLKTTYKAST SKIVSSFKTT TSRAACQLVK EYIGHRDGIW DVSVAKTQPV VLGTASADHT
181ALLWSIETGK CLVKYAGHVG SVNSIKFHPS EQLALTASGD QTAHIWRYAV QLPTPQPVAD
241TSQISGEDEV ECSDKDEPDL DGDVSSDCPT IRVPLTSLKS HQGVVIASDW LVGGKQAVTA
301SWDRTANLYD VETSELVHSL TGHDQELTHC CTHPTQRLVV TSSRDTTFRL WDFRDPSIHS
361VNVFQGHTDT VTSAVFTVGD NVVSGSDDRT VKVWDLKNMR SPIATIRTDS AINRINVCVG
421QKIIALPHDN RQVRLFDMSG VRLARLPRSS RQGHRRMVCC SAWSEDHPVC NLFTCGFDRQ
481AIGWNINIPA LLQEK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

495 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.