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WDR34 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR34 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR34 antibodies →
$504.00each
Catalog Number: LS-G108155-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR34 (NM_052844), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA216B9.3 | RP11-216B9.5 | WD repeat domain 34 | WDR34
Origin Species
Human
SwissProt
Q96EX3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATRAQPGPL SQAGSAGVAA LATVGVASGP GPGRPGPLQD ETLGVASVPS QWRAVQGIRG
61ETKSCQTASI ATASASAQAR NHVDAQVQTE APVPVSVQPP SQYDIPRLAA FLRRVEAMVI
121RELNKNWQSH AFDGFEVNWT EQQQMVSCLY TLGYPPAQAQ GLHVTSISWN STGSVVACAY
181GRLDHGDWST LKSFVCAWNL DRRDLRPQQP SAVVEVPSAV LCLAFHPTQP SHVAGGLYSG
241EVLVWDLSRL EDPLLWRTGL TDDTHTDPVS QVVWLPEPGH SHRFQVLSVA TDGKVLLWQG
301IGVGQLQLTE GFALVMQQLP RSTKLKKHPR GETEVGATAV AFSSFDPRLF ILGTEGGFPL
361KCSLAAGEAA LTRMPSSVPL RAPAQFTFSP HGGPIYSVSC SPFHRNLFLS AGTDGHVHLY
421SMLQAPPLTS LQLSLKYLFA VRWSPVRPLV FAAASGKGDV QLFDLQKSSQ KPTVLIKQTQ
481DESPVYCLEF NSQQTQLLAA GDAQGTVKVW QLSTEFTEQG PREAEDLDCL AAEVAA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

536 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.