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WDR27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR27 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR27 antibodies →
$725.00each
Catalog Number: LS-G104241-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR27 (NM_182552), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_182552

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENPQDIFSS NGGCLSDIVI EKYLVESKES VSHVQLACSM QDCAFPLDGT ELCIWNTKDP
61SHQLLILRGH HQPITAMAFG NKVNPLLICS ASLDYVIMWN LDECREKVLQ GLVPRGTVMG
121SLLGKVLCLQ LSLDDHVVAV CAGNKIFMLD IEQRFSVTYI ERPDVNNRHK VPPPTFLHTF
181SQTQAVRAEL QGHLGPVTAV EFCPWRAGTL ISASEDRGFK VWDHCTGSLI YSSSVLSAYP
241LLSLFIDAES RQLVTGCADG QLWIFSLMDG HHYRRVARVD LRKKTETFST RRVKSGLCSQ
301PEESQLPSTS ALGKGEQVEV TFPVLRLAPC DLSLIPNSAC GCLSSENTRC VWIGSSVGLF
361VFNLANLEVE AALYYKDFQS LSILLAGSCA LRNRTADQKV LCLLASLFGG KIAVLEINPA
421ALVRAQQCPS MGQSLSVPAS SCVLPTSPLY LGIAKEKSTK AASEQRRAAR NVMKDQRLVF
481HSKVRSSGYA SAPHVTMFSP KTNIKSEGKG SSRSRSSCAR EAYPVECAVP TKPGPQVAAA
541PTCTRVCCIQ YSGDGQWLAC GLANHLLLVF DASLTGTPAV FSGHDGAVNA VCWSQDRRWL
601LSAARDGTLR MWSARGAELA LLLGKDMFSK PIQSAQFYYI DAFILLSSGP EFQLLRYHID
661TCKDEIKRYK QKSKSKLICR LSTTGAVDMT SLSAVNDFYS HIVLAAGRNR TVEVFDLNAG
721CSAAVIAEAH SRPVHQICQN KGSSFTTQQP QAYNLFLTTA IGDGMRLWDL RTLRCERHFE
781GHPTRGYPCG IAFSPCGRFA ACGAEDRHAY VYEMGSSTFS HRLAGHTDTV TGVAFNPSAP
841QLATATLDGK LQLFLAE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

857 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.