LSBio
WDR24 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR24 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR24 antibodies →
$504.00each
Catalog Number: LS-G102036-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR24 (NM_032259), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_032259

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
88.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEKMSRVTTA LGGSVLTGRT MHCHLDAPAN AISVCRDAAQ VVVAGRSIFK IYAIEEEQFV
61EKLNLRVGRK PSLNLSCADV VWHQMDENLL ATAATNGVVV TWNLGRPSRN KQDQLFTEHK
121RTVNKVCFHP TEAHVLLSGS QDGFMKCFDL RRKDSVSTFS GQSESVRDVQ FSIRDYFTFA
181STFENGNVQL WDIRRPDRCE RMFTAHNGPV FCCDWHPEDR GWLATGGRDK MVKVWDMTTH
241RAKEMHCVQT IASVARVKWR PECRHHLATC SMMVDHNIYV WDVRRPFVPA AMFEEHRDVT
301TGIAWRHPHD PSFLLSGSKD SSLCQHLFRD ASQPVERANP EGLCYGLFGD LAFAAKESLV
361AAESGRKPYT GDRRHPIFFK RKLDPAEPFA GLASSALSVF ETEPGGGGMR WFVDTAERYA
421LAGRPLAELC DHNAKVAREL GRNQVAQTWT MLRIIYCSPG LVPTANLNHS VGKGGSCGLP
481LMNSFNLKDM APGLGSETRL DRSKGDARSD TVLLDSSATL ITNEDNEETE GSDVPADYLL
541GDVEGEEDEL YLLDPEHAHP EDPECVLPQE AFPLRHEIVD TPPGPEHLQD KADSPHVSGS
601EADVASLAPV DSSFSLLSVS HALYDSRLPP DFFGVLVRDM LHFYAEQGDV QMAVSVLIVL
661GERVRKDIDE QTQEHWYTSY IDLLQRFRLW NVSNEVVKLS TSRAVSCLNQ ASTTLHVNCS
721HCKRPMSSRG WVCDRCHRCA SMCAVCHHVV KGLFVWCQGC SHGGHLQHIM KWLEGSSHCP
781AGCGHLCEYS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

790 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.