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WDR13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR13 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR13 antibodies →
$504.00each
Catalog Number: LS-G112141-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR13 (NM_017883), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
WD repeat domain 13 | MG21 | WDR13
Origin Species
Human
NCBI GenBank
NM_017883
SwissProt
Q9H1Z4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
53.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MAAVWQQVLA VDARYNAYRT PTFPQFRTQY IRRRSQLLRE NAKAGHPPAL RRQYLRLRGQ
61LLGQRYGPLS EPGSARAYSN SIVRSSRTTL DRMEDFEDDP RALGARGHRR SVSRGSYQLQ
121AQMNRAVYED RPPGSVVPTS AAEASRAMAG DTSLSENYAF AGMYHVFDQH VDEAVPRVRF
181ANDDRHRLAC CSLDGSISLC QLVPAPPTVL RVLRGHTRGV SDFAWSLSND ILVSTSLDAT
241MRIWASEDGR CIREIPDPDS AELLCCTFQP VNNNLTVVGN AKHNVHVMNI STGKKVKGGS
301SKLTGRVLAL SFDAPGRLLW AGDDRGSVFS FLFDMATGKL TKAKRLVVHE GSPVTSISAR
361SWVSREARDP SLLINACLNK LLLYRVVDNE GTLQLKRSFP IEQSSHPVRS IFCPLMSFRQ
421GACVVTGSED MCVHFFDVER AAKAAVNKLQ GHSAPVLDVS FNCDESLLAS SDASGMVIVW
481RREQK
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.