Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
WDR11 / BRWD2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111979-100
Catalog Number: LS-G111979
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR11 (NM_018117), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HH14 | WD repeat domain 11 | WD repeat domain 15 | WDR11 | BRWD2 | DR11 | KIAA1351 | WDR15
- Origin Species
- Human
- NCBI GenBank
- NM_018117
- SwissProt
- Q9BZH6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 136.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPYTVNFKV SARTLTGALN AHNKAAVDWG WQGLIAYGCH SLVVVIDSIT AQTLQVLEKH61KADVVKVKWA RENYHHNIGS PYCLRLASAD VNGKIIVWDV AAGVAQCEIQ EHAKPIQDVQ121WLWNQDASRD LLLAIHPPNY IVLWNADTGT KLWKKSYADN ILSFSFDPFD PSHLTLLTSE181GIVFISDFSP SKPPSGPGKK VYISSPHSSP AHNKLATATG AKKALNKVKI LITQEKPSAE241FITLNDCLQL AYLPSKRNHM LLLYPREILI LDLEVNQTVG VIAIERTGVP FLQVIPCFQR301DGLFCLHENG CITLRVRRSY NNIFTTSNEE PDPDPVQELT YDLRSQCDAI RVTKTVRPFS361MVCCPVNENA AALVVSDGRV MIWELKSAVC NRNSRNSSSG VSPLYSPVSF CGIPVGVLQN421KLPDLSLDNM IGQSAIAGEE HPRGSILREV HLKFLLTGLL SGLPAPQFAI RMCPPLTTKN481IKMYQPLLAV GTSNGSVLVY HLTSGLLHKE LSIHSCEVKG IEWTSLTSFL SFATSTPNNM541GLVRNELQLV DLPTGRSIAF RGERGNDESA IEMIKVSHLK QYLAVVFRDK PLELWDVRTC601TLLREMSKNF PTITALEWSP SHNLKSLRKK QLATREAMAR QTVVSDTELS IVESSVISLL661QEAESKSELS QNISAREHFV FTDIDGQVYH LTVEGNSVKD SARIPPDGSM GSITCIAWKG721DTLVLGDMDG NLNFWDLKGR VSRGIPTHRS WVRKIRFAPG KGNQKLIAMY NDGAEVWDTK781EVQMVSSLRS GRNVTFRILD VDWCTSDKVI LASDDGCIRV LEMSMKSACF RMDEQELTEP841VWCPYLLVPR ASLALKAFLL HQPWNGQYSL DISHVDYPEN EEIKNLLQEQ LNSLSNDIKK901LLLDPEFTLL QRCLLVSRLY GDESELHFWT VAAHYLHSLS QEKSASTTAP KEAAPRDKLS961NPLDICYDVL CENAYFQKFQ LERVNLQEVK RSTYDHTRKC TDQLLLLGQT DRAVQLLLET1021SADNQHYYCD SLKACLVTTV TSSGPSQSTI KLVATNMIAN GKLAEGVQLL CLIDKAADAC1081RYLQTYGEWN RAAWLAKVRL NPEECADVLR RWVDHLCSPQ VNQKSKALLV LLSLGCFFSV1141AETLHSMRYF DRAALFVEAC LKYGAFEVTE DTEKLITAIY ADYARSLKNL GFKQGAVLFA1201SKAGAAGKDL LNELESPKEE PIEETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1224 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.