LSBio
WDR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WDR1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WDR1 antibodies →
$504.00each
Catalog Number: LS-G101476-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WDR1 (NM_017491), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AIP1 | Actin-interacting protein 1 | NORI-1 | WD repeat-containing protein 1 | WDR1 | WD repeat domain 1
Origin Species
Human
NCBI GenBank
NM_017491
SwissProt
O75083

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPYEIKKVFA SLPQVERGVS KIIGGDPKGN NFLYTNGKCV ILRNIDNPAL ADIYTEHAHQ
61VVVAKYAPSG FYIASGDVSG KLRIWDTTQK EHLLKYEYQP FAGKIKDIAW TEDSKRIAVV
121GEGREKFGAV FLWDSGSSVG EITGHNKVIN SVDIKQSRPY RLATGSDDNC AAFFEGPPFK
181FKFTIGDHSR FVNCVRFSPD GNRFATASAD GQIYIYDGKT GEKVCALGGS KAHDGGIYAI
241SWSPDSTHLL SASGDKTSKI WDVSVNSVVS TFPMGSTVLD QQLGCLWQKD HLLSVSLSGY
301INYLDRNNPS KPLHVIKGHS KSIQCLTVHK NGGKSYIYSG SHDGHINYWD SETGENDSFA
361GKGHTNQVSR MTVDESGQLI SCSMDDTVRY TSLMLRDYSG QGVVKLDVQP KCVAVGPGGY
421AVVVCIGQIV LLKDQRKCFS IDNPGYEPEV VAVHPGGDTV AIGGVDGNVR LYSILGTTLK
481DEGKLLEAKG PVTDVAYSHD GAFLAVCDAS KVVTVFSVAD GYSENNVFYG HHAKIVCLAW
541SPDNEHFASG GMDMMVYVWT LSDPETRVKI QDAHRLHHVS SLAWLDEHTL VTTSHDASVK
601EWTITYTRTR PLEQKLISEE DLAANDILDY KDDDDKV

637 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.