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WAC Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

WAC Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all WAC antibodies →
$504.00each
Catalog Number: LS-G112553-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human WAC (NM_016628), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BM-016 | KIAA1844 | PRO1741 | WWP4 | WAC
Origin Species
Human
NCBI GenBank
NM_016628
SwissProt
Q9BTA9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
70.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVMYARKQQR LSDGCHDRRG DSQPYQALKY SSKSHPSSGD HRHEKMRDAG DPSPPNKMLR
61RSDSPENKYS DSTGHSKAKN VHTHRVRERD GGTSYSPQEN SHNHSALHSS NSHSSNPSNN
121PSKTSDAPYD SADDWSEHIS SSGKKYYYNC RTEVSQWEKP KEWLEREQRQ KEANKMAVNS
181FPKDRDYRRE VMQATATSGF ASGMEDKHSS DASSLLPQNI LSQTSRHNDR DYRLPRAETH
241SSSTPVQHPI KPVVHPTATP STVPSSPFTL QSDHQPKKSF DANGASTLSK LPTPTSSVPA
301QKTERKESTS GDKPVSHSCT TPSTSSASGL NPTSAPPTSA SAVPVSPVPQ SPIPPLLQDP
361NLLRQLLPAL QATLQLNNSN VDISKINEVL TAAVTQASLQ SIIHKFLTAG PSAFNITSLI
421SQAAQLSTQA QPSNQSPMSL TSDASSPRSY VSPRISTPQT NTVPIKPLIS TPPVSSQPKV
481STPVVKQGPV SQSATQQPVT ADKQQGHEPV SPRSLQRSSS QRSPSPGPNH TSNSSNASNA
541TVVPQNSSAR STCSLTPALA AHFSENLIKH VQGWPADHAE KQASRLREEA HNMGTIHMSE
601ICTELKNLRS LVRVCEIQAT LREQRILFLR QQIKELEKLK SEFLHGVKM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

649 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.