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VWA5B2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VWA5B2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G127333-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VWA5B2 (NM_138345), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_138345

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
131.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPGLYCPSSW TPLPLTDSWV RACANGPCLS VRARLTYRNP QPQPVDGVFV YPLAEAEVVS
61GFEAEAAGRR VSFQLQSRRR SQAACCRALG PGLGTPTPRR CAQGHLVLDL AQARSTLVLP
121TGIIAAAGTM TVTLHSSREL PSRPDGVLHV ALPTVLTPLA PPGPPGPPRP PGLCDDSPTS
181CFGVGSLQEE GLAWEELAAP RDVFSGPARC PAPYTFSFEM LVTGPCLLAG LESPSHALRA
241DAPPHASSAA TICVTLAEGH HCDRALEILL HPSEPHQPHL MLEGGSLSSA EYEARVRARR
301DFQRLQRRDS DGDRQVWFLQ RRFHKDILLN PVLALSFCPD LSSKPGHLGT ATRELLFLLD
361SSSVAHKDAI VLAVKSLPPQ TLINLAVFGT LVQPLFPESR PCSDDAVQLI CESIETLQVP
421SGPPDVLAAL DWAVGQPQHR AYPRQLFLLT AASPMAATTH RTLELMRWHR GTARCFSFGL
481GPTCHQLLQG LSALSRGQAY FLRPGQRLQP MLVQALRKAL EPALSDISVD WFVPDTVEAL
541LTPREIPALY PGDQLLGYCS LFRVDGFRSR PPGGQEPGWQ SSGGSVFPSP EEAPSAASPG
601TEPTGTSEPL GTGTVSAELS SPWAARDSEQ STDALTDPVT DPGPNPSDTA IWRRIFQSSY
661IREQYVLTHC SASPEPGPGS TGSSESPGSQ GPGSPEGSAP LEPPSQQGCR SLAWGEPAGS
721RSCPLPAPTP APFKVGALST EVLGRQHRAA LAGRSLSSPP GRANQVPGRP RKPSLGAILD
781GPSPEPGQQL GQGLDDSGNL LSPAPMDWDM LMEPPFLFTA VPPSGELAPP AVPPQAPRCH
841VVIRGLCGEQ PMCWEVGVGL ETLWGPGDGS QPPSPPVREA AWDQALHRLT AASVVRDNEQ
901LALRGGAETT ADRGHARRCW LRALQTSKVS SAPSCFTCPV AVDATTREVL PGALQVCSSE
961PAEPPGTPPA SHSHLDAAPL PTVVYSKGLQ RGSPAGAWDS DQNGNSKRAL GDPATPTEGP
1021RRPPPRPPCR LSMGRRHKLC SPDPGQANNS EGSDHDYLPL VRLQEAPGSF RLDAPFCAAV
1081RISQERLCRA SPFAVHRASL SPTSASLPWA LLGPGVGQGD SATASCSPSP SSGSEGPGQV
1141DSGRGSDTEA SEGAEGLGGT DLRGRTWATA VALAWLEHRC AAAFDEWELT AAKADCWLRA
1201QHLPDGLDLA ALKAAARGLF LLLRHWDQNL QLHLLCYSPA NV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1242 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.