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VWA5B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VWA5B1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G123778-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VWA5B1 (NM_001039500), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001039500

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPGLLNWITG AALPLTASDV TSCVSGYALG LTASLTYGNL EAQPFQGLFV YPLDECTTVI
61GFEAVIADRV VTVQIKDKAK LESGHFDASH VRSPTVTGNI LQDGVSIAPH SCTPGKVTLD
121EDLERILFVA NLGTIAPMEN VTIFISTSSE LPTLPSGAVR VLLPAVCAPT VPQFCTKSTG
181TSNQQAQGKD RHCFGAWAPG SWNKLCLATL LNTEVSNPME YEFNFQLEIR GPCLLAGVES
241PTHEIRADAA PSARSAKSII ITLANKHTFD RPVEILIHPS EPHMPHVLIE KGDMTLGEFD
301QHLKGRTDFI KGMKKKSRAE RKTEIIRKRL HKDIPHHSVI MLNFCPDLQS VQPCLRKAHG
361EFIFLIDRSS SMSGISMHRV KDAMLVALKS LMPACLFNII GFGSTFKSLF PSSQTYSEDS
421LAMACDDIQR MKADMGGTNI LSPLKWVIRQ PVHRGHPRLL FVITDGAVNN TGKVLELVRN
481HAFSTRCYSF GIGPNVCHRL VKGLASVSEG SAELLMEGER LQPKMVKSLK KAMAPVLSDV
541TVEWIFPETT EVLVSPVSAS SLFPGERLVG YGIVCDASLH ISNPRSDKRR RYSMLHSQES
601GSSVFYHSQD DGPGLEGGDC AKNSGAPFIL GQAKNARLAS GDSTTKHDLN LSQRRRAYST
661NQITNHKPLP RATMASDPMP AAKRYPLRKA RLQDLTNQTS LDVQRWQIDL QPLLNSGQDL
721NQGPKLRGPG ARRPSLLPQG CQPFLPWGQE TQAWSPVRER TSDSRSPGDL EPSHHPSAFE
781TETSSDWDPP AESQERASPS RPATPAPVLG KALVKGLHDS QRLQWEVSFE LGTPGPERGG
841AQDADLWSET FHHLAARAII RDFEQLAERE GEIEQGSNRR YQVSALHTSK ACNIISKYTA
901FVPVDVSKSR YLPTVVEYPN SGAALRMLGS RALAQQWRGT SSGFGRPQTM LGEDSAPGND
961MEASPTALFS EARSPGREKH GASEGPQRSL ATNTLSSMKA SENLFGSWLN LNKSRLLTRA
1021AKGFLSKPLI KAVESTSGNQ SFDYIPLVSL QLASGAFLLN EAFCEATHIP MEKLKWTSPF
1081TCHRVSLTTR PSESKTPSPQ LCTSSPPRHP SCDSFSLEPL AKGKLGLEPR AVVEHTGKLW
1141ATVVGLAWLE HSSASYFTEW ELVAAKANSW LEQQEVPEGR TQGTLKAAAR QLFVLLRHWD
1201ENLEFNMLCY NPNYV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1215 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.