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VWA5A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VWA5A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VWA5A antibodies →
$725.00each
Catalog Number: LS-G101238-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Breast cancer suppressor candidate 1(VWA5A) (NM_014622), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BCSC-1 | LOH11CR2A | VWA5A | Ortholog of mouse AW551984 | BCSC1
Origin Species
Human
NCBI GenBank
NM_014622
SwissProt
O00534

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVHFCGLLTL HREPVPLKSI SVSVNIYEFV AGVSATLNYE NEEKVPLEAF FVFPMDEDSA
61VYSFEALVDG KKIVAELQDK MKARTNYEKA ISQGHQAFLL EGDSSSRDVF SCNVGNLQPG
121SKAAVTLKYV QELPLEADGA LRFVLPAVLN PRYQFSGSSK DSCLNVKTPI VPVEDLPYTL
181SMVATIDSQH GIEKVQSNCP LSPTEYLGED KTSAQVSLAA GHKFDRDVEL LIYYNEVHTP
241SVVLEMGMPN MKPGHLMGDP SAMVSFYPNI PEDQPSNTCG EFIFLMDRSG SMQSPMSSQD
301TSQLRIQAAK ETLILLLKSL PIGCYFNIYG FGSSYEACFP ESVKYTQQTM EEALGRVKLM
361QADLGGTEIL APLQNIYRGP SIPGHPLQLF VFTDGEVTDT FSVIKEVRIN RQKHRCFSFG
421IGEGTSTSLI KGIARASGGT SEFITGKDRM QSKALRTLKR SLQPVVEDVS LSWHLPPGLS
481AKMLSPEQTV IFRGQRLISY AQLTGRMPAA ETTGEVCLKY TLQGKTFEDK VTFPLQPKPD
541VNLTIHRLAA KSLLQTKDMG LRETPASDKK DALNLSLESG VISSFTAFIA INKELNKPVQ
601GPLAHRDVPR PILLGASAPL KIKCQSGFRK ALHSDRPPSA SQPRGELMCY KAKTFQMDDY
661SLCGLISHKD QHSPGFGENH LVQLIYHQNA NGSWDLNEDL AKILGMSLEE IMAAQPAELV
721DSSGWATILA VIWLHSNGKD LKCEWELLER KAVAWMRAHA GSTMPSVVKA AITFLKSSVD
781PAIFAF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

786 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.