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VWA3A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VWA3A Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129164-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VWA3A (NM_173615), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
VWA3A
Origin Species
Human
NCBI GenBank
NM_173615
SwissProt
A6NCI4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
133.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKKYRKISIG CFAMATQTSH VFHGQENMFL ENHCIRRNTG RDSKKPLKQK NMNGLGQNSD
61NGLLVTHVNQ TQDLLRLQGS ETQSSDWEDS EDWLSAHSLK CQKLTLADLI SQGTEVLEEG
121TNVVQKICFS TQIIRHFESK LSDTIEVYQE RIQWLTENSK KAFGLIKGAR VSILIDVSAI
181SSGPQKEEFQ KDLMSLIDEQ LSHKEKLFVL SFGTNAGSLW PDPMEVSAST LQELKLWVKT
241LQPDGGSNLL QALKKIFTLK GLDSLVAIMR SCPDQPSEIL SDYIQQSTMG RDLIIHFITY
301RCDDQMPPAV LKNLAEAVRG YYHCYSPKME HYTSRDMDEL LAEIQKAQSL LSHVQALQHS
361SPCEALTCTM EEISTEITNG PLISLLPKPP KHDAPLTIEF PNLDKTSAEW LKVNGLKAKK
421LSLYQVLAPN AFSPVEEFVP ILQKTVSSTI HEKAMIQFEW HDGTVKNIHV DPPFLYKYQQ
481QLSRAMRMYE RRIEWLSLAS RRIWGTVCEK RVVVLLDISA TNSMYIIHIQ HSLRLLLEEQ
541LSNKDCFNLI AFGSTIESWR PEMVPVSHNN LQSAWRWALN LRCRGSRNVL SALRKAVEVD
601FKDKDKHQSQ GIYLFTGGIP DQDMPTLSAY MAEACGGCDL QLNVCLFYVG EPKMDTTPPA
661RYASHTDTAA AYKEVTRAAG GRFHWFGDTG IYESDDINSI MSEMEKALNY SQKCAFLMAS
721LKNHSGKVLG SSALPKEKPK TLQLRSQPKK LCPPRPTVPL GARMSIKDDP DREKSPPLKS
781LKWRPLSSRV GISPAAAQPT KEGMMELRRK TKSREAETSL LLFYTEKGND VGSVYKKYPQ
841GRGLRRTSSS IDLPRKDTVC SSQEWVAKYG LKKLKLEISR CMGPNCTHQK SGQRSASAKH
901CSIFPSVEIH GVVRHIQWTP REMEVYIRHL EKVLRRYVQR LQWLLSGSRR LFGTVLESKV
961CILLDTSGSM GPYLQQVKTE LVLLIWEQLR KCCDSFNLLS FAESFQSWQD TLVETTDAAC
1021HEAMQWVTHL QAQGSTSILQ ALLKAFSFHD LEGLYLLTDG KPDTSCSLVL NEVQKLREKR
1081DVKVHTISLN CSDRAAVEFL RKLASFTGGR YHCPVGEDTL SKIHSLLTKG FINEKDPTLP
1141PFEGDDLRIL AQEITKARSF LWQAQSFRSQ LQKKNDAEPK VTLS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1184 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.