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VSIG10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VSIG10 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VSIG10 antibodies →
$504.00each
Catalog Number: LS-G111449-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VSIG10 (NM_019086), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
VSIG10
Origin Species
Human
NCBI GenBank
NM_019086
SwissProt
Q8N0Z9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGGSAPEP RVLVCLGALL AGWVAVGLEA VVIGEVHENV TLHCGNISGL RGQVTWYRNN
61SEPVFLLSSN SSLRPAEPRF SLVDATSLHI ESLSLGDEGI YTCQEILNVT QWFQVWLQVA
121SGPYQIEVHI VATGTLPNGT LYAARGSQVD FSCNSSSRPP PVVEWWFQAL NSSSESFGHN
181LTVNFFSLLL ISPNLQGNYT CLALNQLSKR HRKVTTELLV YYPPPSAPQC WAQMASGSFM
241LQLTCRWDGG YPDPDFLWIE EPGGVIVGKS KLGVEMLSES QLSDGKKFKC VTSHIVGPES
301GASCMVQIRG PSLLSEPMKT CFTGGNVTLT CQVSGAYPPA KILWLRNLTQ PEVIIQPSSR
361HLITQDGQNS TLTIHNCSQD LDEGYYICRA DSPVGVREME IWLSVKEPLN IGGIVGTIVS
421LLLLGLAIIS GLLLHYSPVF CWKVGNTSRG QNMDDVMVLV DSEEEEEEEE EEEEDAAVGE
481QEGAREREEL PKEIPKQDHI HRVTALVNGN IEQMGNGFQD LQDDSSEEQS DIVQEEDRPV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

540 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.