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VRTN Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VRTN Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VRTN antibodies →
$504.00each
Catalog Number: LS-G111886-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VRTN (NM_018228), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C14orf115 | VRTN | Vertnin
Origin Species
Human
NCBI GenBank
NM_018228
SwissProt
Q9H8Y1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSRNQLVQK VLQELQEAVE CEGLEGLIGA SLEAKQVLSS FTLPTCREGG PGLQVLEVDS
61VALSLYPEDA PRNMLPLVCK GEGSLLFEAA SMLLWGDAGL SLELRARTVV EMLLHRHYYL
121QGMIDSKVML QAVRYSLCSE ESPEMTSLPP ATLEAIFDAD VKASCFPSSF SNVWHLYALA
181SVLQRNIYSI YPMRNLKIRP YFNRVIRPRR CDHVPSTLHI MWAGQPLTSH FFRHQYFAPV
241VGLEEVEAEG APGVAPALPA LAPLSSPAKT LELLNREPGL SYSHLCERYS VTKSTFYRWR
301RQSQEHRQKV AARFSAKHFL QDSFHRGGVV PLQQFLQRFP EISRSTYYVW KHELLGSGTC
361PALPPREVLG MEELEKLPEE QVAEEELECS ALAVSSPGMV LMQRAKLYLE HCISLNTLVP
421YRCFKRRFPG ISRSTYYNWR RKALRRNPSF KPAPALSAAG TPQLASVGEG AVIPWKSEAE
481EGAGNATGED PPAPGELLPL RMPLSRWQRR LRRAARRQVL SGHLPFCRFR LRYPSLSPSA
541FWVWKSLARG WPRGLSKLQV PVPTLGKGGQ EAEEKQEKEA GRDVTAVMAP PVGASSEDVE
601GGPSREGALQ EGATAQGQPH SGPLLSQPVV AAAGGRDGRM LVMDMIATTK FKAQAKLFLQ
661KRFQSKSFPS YKEFSALFPL TARSTYYMWK RALYDGLTLV DG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

702 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.