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VPS41 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VPS41 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VPS41 antibodies →
$725.00each
Catalog Number: LS-G102196-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPS41 (NM_080631), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HVPS41 | HVSP41 | HVps41p | S53 | Vacuolar assembly protein 41 | VPS41
Origin Species
Human
NCBI GenBank
NM_014396
SwissProt
P49754

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
95.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MAEAEEQETG SLEESTDESE EEESEEEPKL KYERLSNGVT EILQKDAASC MTVHDKFLAL
61GTHYGKVYLL DVQGNITQKF DVVQVFGLYS GEEFHETFDC PIKIIAVHPH FVRSSCKQFV
121TGGKKLLLFE RSWMNRWKSA VLHEGEGNIR SVKWRGHLIA WANNMGVKIF DIISKQRITN
181VPRDDISLRP DMYPCSLCWK DNVTLIIGWG TSVKVCSVKE RHASEMRDLP SRYVEIVSQF
241ETEFYISGLA PLCDQLVVLS YVKEISEKTE REYCARPRLD IIQPLSETCE EISSDALTVR
301GFQENECRDY HLEYSEGESL FYIVSPRDVV VAKERDQDDH IDWLLEKKKY EEALMAAEIS
361QKNIKRHKIL DIGLAYINHL VERGDYDIAA RKCQKILGKN AALWEYEVYK FKEIGQLKAI
421SPYLPRGDPV LKPLIYEMIL HEFLESDYEG FATLIREWPG DLYNNSVIVQ AVRDHLKKDS
481QNKTLLKTLA ELYTYDKNYG NALEIYLTLR HKDVFQLIHK HNLFSSIKDK IVLLMDFDSE
541KAVDMLLDNE DKISIKKVVE ELEDRPELQH VYLHKLFKRD HHKGQRYHEK QISLYAEYDR
601PNLLPFLRDS THCPLEKALE ICQQRNFVEE TVYLLSRMGN SRSALKMIME ELHDVDKAIE
661FAKEQDDGEL WEDLILYSID KPPFITGLLN NIGTHVDPIL LIHRIKEGME IPNLRDSLVK
721ILQDYNLQIL LREGCKKILV ADSLSLLKKM HRTQMKGVLV DEENICESCL SPILPSDAAK
781PFSVVVFHCR HMFHKECLPM PSMNSAAQFC NICSAKNRGP GSAILEMKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

829 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.