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VPS39 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VPS39 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113344-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPS39 (NM_015289), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HVam6p | KIAA0770 | TRAP1-like protein | Vam6/Vps39-like protein | VAM6 | VPS39 | TLP
Origin Species
Human
SwissProt
Q96JC1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHDAFEPVPI LEKLPLQIDC LAAWEEWLLV GTKQGHLLLY RIRKDVGCNR FEVTLEKSNK
61NFSKKIQQIH VVSQFKILVS LLENNIYVHD LLTFQQITTV SKAKGASLFT CDLQHTETGE
121EVLRMCVAVK KKLQLYFWKD REFHELQGDF SVPDVPKSMA WCENSICVGF KRDYYLIRVD
181GKGSIKELFP TGKQLEPLVA PLADGKVAVG QDDLTVVLNE EGICTQKCAL NWTDIPVAME
241HQPPYIIAVL PRYVEIRTFE PRLLVQSIEL QRPRFITSGG SNIIYVASNH FVWRLIPVPM
301ATQIQQLLQD KQFELALQLA EMKDDSDSEK QQQIHHIKNL YAFNLFCQKR FDESMQVFAK
361LGTDPTHVMG LYPDLLPTDY RKQLQYPNPL PVLSGAELEK AHLALIDYLT QKRSQLVKKL
421NDSDHQSSTS PLMEGTPTIK SKKKLLQIID TTLLKCYLHT NVALVAPLLR LENNHCHIEE
481SEHVLKKAHK YSELIILYEK KGLHEKALQV LVDQSKKANS PLKGHERTVQ YLQHLGTENL
541HLIFSYSVWV LRDFPEDGLK IFTEDLPEVE SLPRDRVLGF LIENFKGLAI PYLEHIIHVW
601EETGSRFHNC LIQLYCEKVQ GLMKEYLLSF PAGKTPVPAG EEEGELGEYR QKLLMFLEIS
661SYYDPGRLIC DFPFDGLLEE RALLLGRMGK HEQALFIYVH ILKDTRMAEE YCHKHYDRNK
721DGNKDVYLSL LRMYLSPPSI HCLGPIKLEL LEPKANLQAA LQVLELHHSK LDTTKALNLL
781PANTQINDIR IFLEKVLEEN AQKKRFNQVL KNLLHAEFLR VQEERILHQQ VKCIITEEKV
841CMVCKKKIGN SAFARYPNGV VVHYFCSKEV NPADT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

875 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.