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VPS37C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VPS37C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VPS37C antibodies →
$504.00each
Catalog Number: LS-G112077-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPS37C (NM_017966), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HVps37C | PML39 | VPS37C | Vacuolar protein sorting 37C | ESCRT-I complex subunit VPS37C
Origin Species
Human
NCBI GenBank
NM_017966
SwissProt
A5D8V6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
38.5 kda
Protein Type
Lysate
Amino Acid Sequence
1METLKDKTLQ ELEELQNDSE AIDQLALESP EVQDLQLERE MALATNRSLA ERNLEFQGPL
61EISRSNLSDR YQELRKLVER CQEQKAKLEK FSSALQPGTL LDLLQVEGMK IEEESEAMAE
121KFLEGEVPLE TFLENFSSMR MLSHLRRVRV EKLQEVVRKP RASQELAGDA PPPRPPPPVR
181PVPQGTPPVV EEQPQPPLAM PPYPLPYSPS PSLPVGPTAH GALPPAPFPV VSQPSFYSGP
241LGPTYPAAQL GPRGAAGYSW SPQRSMPPRP GYPGTPMGAS GPGYPLRGGR APSPGYPQQS
301PYPATGGKPP YPIQPQLPSF PGQPQPSVPL QPPYPPGPAP PYGFPPPPGP AWPGY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

355 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.