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VPS37B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VPS37B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109873-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPS37B (NM_024667), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ESCRT-I complex subunit VPS37B | Vacuolar protein sorting 37B | HVps37B | VPS37B
Origin Species
Human
NCBI GenBank
NM_024667
SwissProt
Q9H9H4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
31.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGAGSEARF AGLSLVQLNE LLEDEGQLTE MVQKMEETQN VQLNKEMTLA SNRSLAEGNL
61LYQPQLDTLK ARLTQKYQEL QVLFEAYQIK KTKLDRQSSS ASLETLLALL QAEGAKIEED
121TENMAEKFLD GELPLDSFID VYQSKRKLAH MRRVKIEKLQ EMVLKGQRLP QALAPLPPRL
181PELAPTAPLP YPAPEASGPP AVAPRRIPPP PPPVPAGRLA TPFTAAMSSG QAVPYPGLQC
241PPLPPRVGLP TQQGFSSQFV SPYPPPLPQR PPPRLPPHQP GFILQTRTRP LEQKLISEED
301LAANDILDYK DDDDKV

316 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.