Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
VPAC2 / VIPR2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100038-100
Catalog Number: LS-G100038
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VPAC2(VIPR2) (NM_003382), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C16DUPq36.3 | DUP7q36.3 | PACAP-R-3 | Pvr3 | Vip type-2 receptor | Vip-r2 | Vip2 receptor | PACAP type III receptor | Pacap type-3 | PACAP-R3 | PACAPR-3 | VIP and PACAP receptor 2 | VIP-R-2 | VIP2R | VIPR2 | VPAC2 | VPCAP2R | PACAP3 | Vip2 | VPAC2R
- Origin Species
- Human
- NCBI GenBank
- NM_003382
- SwissProt
- P41587
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 49.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRTLLPPALL TCWLLAPVNS IHPECRFHLE IQEEETKCAE LLRSQTEKHK ACSGVWDNIT61CWRPANVGET VTVPCPKVFS NFYSKAGNIS KNCTSDGWSE TFPDFVDACG YSDPEDESKI121TFYILVKAIY TLGYSVSLMS LATGSIILCL FRKLHCTRNY IHLNLFLSFI LRAISVLVKD181DVLYSSSGTL HCPDQPSSWV GCKLSLVFLQ YCIMANFFWL LVEGLYLHTL LVAMLPPRRC241FLAYLLIGWG LPTVCIGAWT AARLYLEDTG CWDTNDHSVP WWVIRIPILI SIIVNFVLFI301SIIRILLQKL TSPDVGGNDQ SQYKRLAKST LLLIPLFGVH YMVFAVFPIS ISSKYQILFE361LCLGSFQGLV VAVLYCFLNS EVQCELKRKW RSRCPTPSAS RDYRVCGSSF SRNGSEGALQ421FHRGSRAQSF LQTETSVITRTRPLEQKLISEEDLAANDILDYKDDDDKV
438 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.