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VNN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VNN2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G107897-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VNN2 (NM_078488), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FOAP-4 | GPI-80 | Pantetheinase | Protein FOAP-4 | Vanin-2 | VNN2 | Vanin 2 | Vannin 2
Origin Species
Human
NCBI GenBank
AJ132100
SwissProt
O95498

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNENIDILET AIKQAAEQGA RIIVTPEDAL YGWKFTRETV FPYLEDIPDP QVNWIPCQDP
61HRFGHTPVQA RLSCLAKDNS IYVLANLGDK KPCNSRDSTC PPNGYFQYNT NVVYNTEGKL
121VARYHKYHLY SEPQFNVPEK PELVTFNTAF GRFGIFTCFD IFFYDPGVTL VKDFHVDTIL
181FPTAWMNVLP LLTAIEFHSA WAMGMGVNLL VANTHHVSLN MTGSGIYAPN GPKVYHYDMK
241TELGKLLLSE VDSHPLSSLA YPTAVNWNAY ATTIKPFPVQ KNTFRGFISR DGFNFTELFE
301NAGNLTVCQK ELCCHLSYRM LQKEENEVYV LGAFTGLHGR RRREYWQVCT MLKCKTTNLT
361TCGRPVETAS TRFEMFSLSG TFGTEYVFPE VLLTEIHLSP GKFEVLKDGR LVNKNGSSGP
421ILTVSLFGRW YTKDSLYSSC GTSNSAITYL LIFILLMIIA LQNIVML
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

467 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.