Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
VMD2L3 / BEST3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106263-100
Catalog Number: LS-G106263
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Bestrophin 3(BEST3) (NM_152439), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BEST3 | Bestrophin-3 | VMD2L3 | Bestrophin 3
- Origin Species
- Human
- NCBI GenBank
- NM_032735
- SwissProt
- Q8N1M1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTTDERKLFN HLKSPHLKYW VPFIWFGNLA TKARNEGRIR DSVDLQSLMT EMNRYRSWCS61LLFGYDWVGI PLVYTQVAEQ LINPFGEDDD DFETNWCIDR NLQVSLLAVD EMHMSLPKMK121KDIYWDDSAA RPPYTLAAAD YCIPSFLGST VQMGLSGSDF PDEEWLWDYE KHGHRHSMIR181RVKRFLSAHE HPSSPRRRSY RRQTSDSSMF LPRDDLSPAR DLLDVPSRNP PRASPTWKKS241CFPEGSPTLH FSMGELSTIR ETSQTSTLQS LTPQSSVRTS PIKMPLVPEV LITAAEAPVP301TSGGYHHDSA TSILSSEFTG VQPSKTEQQQ GPMGSILSPS EKETPPGGPS PQTVSASAEE361NIFNCEEDPG DTFLKRWSLP GFLGSSHTSL GNLSPDPMSS QPALLIDTET SSEISGINIV421AGSRVSSDML YLMENLDTKE TDIIELNKET EESPKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
455 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.