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VGF Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VGF Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all VGF antibodies →
$504.00each
Catalog Number: LS-G117388-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VGF (NM_003378), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Neurosecretory protein VGF | VGF
Origin Species
Human
NCBI GenBank
NM_003378
SwissProt
O15240

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.26 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKALRLSASA LFCLLLINGL GAAPPGRPEA QPPPLSSEHK EPVAGDAVPG PKDGSAPEVR
61GARNSEPQDE GELFQGVDPR ALAAVLLQAL DRPASPPAPS GSQQGPEEEA AEALLTETVR
121SQTHSLPAPE SPEPAAPPRP QTPENGPEAS DPSEELEALA SLLQELRDFS PSSAKRQQET
181AAAETETRTH TLTRVNLESP GPERVWRASW GEFQARVPER APLPPPAPSQ FQARMPDSGP
241LPETHKFGEG VSSPKTHLGE ALAPLSKAYQ GVAAPFPKAR RPESALLGGS EAGERLLQQG
301LAQVEAGRRQ AEATRQAAAQ EERLADLASD LLLQYLLQGG ARQRGLGGRG LQEAAEERES
361AREEEEAEQE RRGGEERVGE EDEEAAEAEA EAEEAERARQ NALLFAEEED GEAGAEDKRS
421QEETPGHRRK EAEGTEEGGE EEDDEEMDPQ TIDSLIELST KLHLPADDVV SIIEEVEEKR
481KRKKNAPPEP VPPPRAAPAP THVRSPQPPP PAPAPARDEL PDWNEVLPPW DREEDEVYPP
541GPYHPFPNYI RPRTLQPPSA LRRRHYHHAL PPSRHYPGRE AQARRAQEEA EAEERRLQEQ
601EELENYIEHV LLRRP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.