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VEPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VEPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101897-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VEPH1 (NM_024621), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
94.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHQLFRLVLG QKDLSRAGDL FSLDDSEIED SLTEALEQIK IISSSSDYQT NNNDQAVVEI
61CITRITTAIR ETESIEKHAK ALVGLWDSCL EHNLRPFGKD EDTPHAKIAS DIMSCILQNY
121NRPPVMALAI PIAVKFLHRG NKELCRNMSN YLSLAAITKA DLLADHTEVI VKSILQGNTM
181LLRVLPAVYE KQPQPINRHL TELLALMSQL EQPEQYHLLR LLHVAAKKKQ LEVVQKCIPF
241LIGHLKDSTH NDIILNILIE IAVYEPVALN SFLPMLKEIG ERFPYLTGQM ARIYGAVGHV
301DEERARSCLT YLVSQLANME HSFHHILLLE IKSITDTFSS ILGPQSRDIF RMSNSFTAIA
361KLLTRQLENT KAGSGRRKIS TEIEFPEKLE ETKLIVTENE DHEKLQVKIQ AFEDKINAGS
421NTPGSIRRYS LGQVSKEERK NIRFNRSKSL AFHTMLTKGV GSDDGEDENR GDIPASISLS
481EIDPLGQGND KLPFKTDTER SQLGESSVSY PNIIHIDSEN LPETVKENSQ EETPETTASP
541IEYQDKLYLH LKKNLSKVKA YAMEIGKKIP VPDQCTIEDT VRSCVAKLFF TCSLKGHYCL
601YSKSSFILIS QEPQPWIQIM FLFQQSLFPE PLSIQSHSVQ FLRALWEKTQ AGGAHSFETA
661MMESTFPQQK DLDQVQLHLE EVRFFDVFGF SETAGAWQCF MCNNPEKATV VNQDGQPLIE
721GKLKEKQVRW KFIKRWKTRY FTLAGNQLLF QKGKSKDDPD DCPIELSKVQ SVKAVAKKRR
781DRSLPRAFEI FTDNKTYVFK AKDEKNAEEW LQCINVAVAQ AKERESREVT TYL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

833 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.