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VAV2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

VAV2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G117382-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VAV2 (NM_003371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
VAV2 | Vav 2 oncogene | VAV-2
Origin Species
Human
NCBI GenBank
NM_003371
SwissProt
P52735

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEQWRQCGRW LIDCKVLPPN HRVVWPSAVV FDLAQALRDG VLLCQLLHNL SPGSIDLKDI
61NFRPQMSQFL CLKNIRTFLK VCHDKFGLRN SELFDPFDLF DVRDFGKVIS AVSRLSLHSI
121AQNKGIRPFP SEETTENDDD VYRSLEELAD EHDLGEDIYD CVPCEDGGDD IYEDIIKVEV
181QQPMKMGMTE DDKRNCCLLE IQETEAKYYR TLEDIEKNYM SPLRLVLSPA DMAAVFINLE
241DLIKVHHSFL RAIDVSVMVG GSTLAKVFLD FKERLLIYGE YCSHMEHAQN TLNQLLASRE
301DFRQKVEECT LKVQDGKFKL QDLLVVPMQR VLKYHLLLKE LLSHSAERPE RQQLKEALEA
361MQDLAMYINE VKRDKETLRK ISEFQSSIEN LQVKLEEFGR PKIDGELKVR SIVNHTKQDR
421YLFLFDKVVI VCKRKGYSYE LKEIIELLFH KMTDDPMNNK DVKKWSYGFY LIHLQGKQGF
481QFFCKTEDMK RKWMEQFEMA MSNIKPDKAN ANHHSFQMYT FDKTTNCKAC KMFLRGTFYQ
541GYMCTKCGVG AHKECLEVIP PCKFTSPADL DASGAGPGPK MVAMQNYHGN PAPPGKPVLT
601FQTGDVLELL RGDPESPWWE GRLVQTRKSG YFPSSSVKPC PVDGRPPISR PPSREIDYTA
661YPWFAGNMER QQTDNLLKSH ASGTYLIRER PAEAERFAIS IKFNDEVKHI KVVEKDNWIH
721ITEAKKFDSL LELVEYYQCH SLKESFKQLD TTLKYPYKSR ERSASRASSR SPVFTPRVIG
781TAVARYNFAA RDMRELSLRE GDVVRIYSRI GGDQGWWKGE TNGRIGWFPS TYVEEEGIQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

839 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.