Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
VASN / Vasorin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107401-100
Catalog Number: LS-G107401
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLITL2(VASN) (NM_138440), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein slit-like 2 | Slit-like 2 | VASN | Vasorin | Slit-like 2 (Drosophila) | SLITL2
- Origin Species
- Human
- SwissProt
- Q6EMK4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MCSRVPLLLP LLLLLALGPG VQGCPSGCQC SQPQTVFCTA RQGTTVPRDV PPDTVGLYVF61ENGITMLDAG SFAGLPGLQL LDLSQNQIAS LPSGVFQPLA NLSNLDLTAN RLHEITNETF121RGLRRLERLY LGKNRIRHIQ PGAFDTLDRL LELKLQDNEL RALPPLRLPR LLLLDLSHNS181LLALEPGILD TANVEALRLA GLGLQQLDEG LFSRLRNLHD LDVSDNQLER VPPVIRGLRG241LTRLRLAGNT RIAQLRPEDL AGLAALQELD VSNLSLQALP GDLSGLFPRL RLLAAARNPF301NCVCPLSWFG PWVRESHVTL ASPEETRCHF PPKNAGRLLL ELDYADFGCP ATTTTATVPT361TRPVVREPTA LSSSLAPTWL SPTAPATEAP SPPSTAPPTV GPVPQPQDCP PSTCLNGGTC421HLGTRHHLAC LCPEGFTGLY CESQMGQGTR PSPTPVTPRP PRSLTLGIEP VSPTSLRVGL481QRYLQGSSVQ LRSLRLTYRN LSGPDKRLVT LRLPASLAEY TVTQLRPNAT YSVCVMPLGP541GRVPEGEEAC GEAHTPPAVH SNHAPVTQAR EGNLPLLIAP ALAAVLLAAL AAVGAAYCVR601RGRAMAAAAQ DKGQVGPGAG PLELEGVKVP LEPGPKATEG GGEALPSGSE CEVPLMGFPG661PGLQSPLHAK PYITRTRPLEQKLISEEDLAANDILDYKDDDDKV
673 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.