Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
VANGL2 / LTAP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111247-100
Catalog Number: LS-G111247
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VANGL2 (NM_020335), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1215 | Loop-tail-associated protein | LTAP | STBM | Loop-tail protein 1 homolog | LPP1 | STB1 | STBM1 | Strabismus 1 | Vang-like protein 2 | Van Gogh-like protein 2 | VANGL2
- Origin Species
- Human
- NCBI GenBank
- AB033041
- SwissProt
- Q9ULK5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTESQYSGY SYKSGHSRSS RKHRDRRDRH RSKSRDGGRG DKSVTIQAPG EPLLDNESTR61GDERDDNWGE TTTVVTGTSE HSISHDDLTR IAKDMEDSVP LDCSRHLGVA AGATLALLSF121LTPLAFLLLP PLLWREELEP CGTACEGLFI SVAFKLLILL LGSWALFFRR PKASLPRVFV181LRALLMVLVF LLVVSYWLFY GVRILDARER SYQGVVQFAV SLVDALLFVH YLAVVLLELR241QLQPQFTLKV VRSTDGASRF YNVGHLSIQR VAVWILEKYY HDFPVYNPAL LNLPKSVLAK301KVSGFKVYSL GEENSTNNST GQSRAVIAAA ARRRDNSHNE YYYEEAEHER RVRKRRARLV361VAVEEAFTHI KRLQEEEQKN PREVMDPREA AQAIFASMAR AMQKYLRTTK QQPYHTMESI421LQHLEFCITH DMTPKAFLER YLAAGPTIQY HKERWLAKQW TLVSEEPVTN GLKDGIVFLL481KRQDFSLVVS TKKVPFFKLS EEFVDPKSHK FVMRLQSETS VTRTRPLEQKLISEEDLAANDILDYKDDDDKV
521 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.