Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
VAC14 / TRX Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112037-100
Catalog Number: LS-G112037
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VAC14 (NM_018052), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ArPIKfyve | Protein VAC14 homolog | TAX1BP2 | TRX | Vac14 homolog (S. cerevisiae) | Tax1-binding protein 2 | VAC14
- Origin Species
- Human
- NCBI GenBank
- NM_018052
- SwissProt
- Q08AM6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 87.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNPEKDFAPL TPNIVRALND KLYEKRKVAA LEIEKLVREF VAQNNTVQIK HVIQTLSQEF61ALSQHPHSRK GGLIGLAACS IALGKDSGLY LKELIEPVLT CFNDADSRLR YYACEALYNI121VKVARGAVLP HFNVLFDGLS KLAADPDPNV KSGSELLDRL LKDIVTESNK FDLVSFIPLL181RERIYSNNQY ARQFIISWIL VLESVPDINL LDYLPEILDG LFQILGDNGK EIRKMCEVVL241GEFLKEIKKN PSSVKFAEMA NILVIHCQTT DDLIQLTAMC WMREFIQLAG RVMLPYSSGI301LTAVLPCLAY DDRKKSIKEV ANVCNQSLMK LVTPEDDELD ELRPGQRQAE PTPDDALPKQ361EGTASGGPDG SCDSSFSSGI SVFTAASTER APVTLHLDGI VQVLNCHLSD TAIGMMTRIA421VLKWLYHLYI KTPRKMFRHT DSLFPILLQT LSDESDEVIL KDLEVLAEIA SSPAGQTDDP481GPLDGPDLQA SHSELQVPTP GRAGLLNTSG TKGLECSPST PTMNSYFYKF MINPSQEIQQ541RTEAPGGQRP FHHQAAVPPA ECGRTSSTQW QTSCCGEEDP QVRLDHGPRP QHHPADLPQE601LFQLRNQLKD LKTLESQNLF CCLYRSWCHN PVTTVSLCFL TQNYRHAYDL IQKFGDLEVT661VDFLAEVDKL VQLIECPIFT YLRLQLLDVK NNPYLIKALY GLLMLLPQSS AFQLLSHRLQ721CVPNPELLQT EDSLKAAPKS QKADSPSIDY AELLQHFEKV QNKHLEVRHQ RSGRGDHLDR781RVVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
784 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.