Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
V1RL1 / VN1R1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G111109-100
Catalog Number: LS-G111109
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human VN1R1 (NM_020633), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HGPCR24 | V3r-related gene protein | V1RL1 | ZVNH1 | V1r-like receptor 1 | VN1R1 | ZVNR1 | V1R-like 1 | VNR19I1 | Vomeronasal 1 receptor 1 | Vomeronasal type-1 receptor 1
- Origin Species
- Human
- NCBI GenBank
- NM_020633
- SwissProt
- Q9GZP7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVGDTLKLLS PLMTRYFFLL FYSTDSSDLN ENQHPLDFDE MAFGKVKSGI SFLIQTGVGI61LGNSFLLCFY NLILFTGHKL RPTDLILSQL ALANSMVLFF KGIPQTMAAF GLKYLLNDTG121CKFVFYYHRV GTRVSLSTIC LLNGFQAIKL NPSICRWMEI KIRSPRFIDF CCLLCWAPHV181LMNASVLLLV NGPLNSKNSS AKNNYGYCSY KASKRFSSLH AVLYFSPDFM SLGFMVWASG241SMVFFLYRHK QQVQHNHSNR LSCRPSQEAR ATHTIMVLVS SFFVFYSVHS FLTIWTTVVA301NPGQWIVTNS VLVASCFPAR SPFVLIMSDT HISQFCFACR TRKTLFPNLV VMPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
353 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.