Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UTP3 / CRLZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101660-100
Catalog Number: LS-G101660
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UTP3 (NM_020368), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CRLZ1 | CRL1 | UTP3 homolog | SAS10 | Disrupter of silencing 10 | Disrupter of silencing SAS10 | UTP3
- Origin Species
- Human
- NCBI GenBank
- NM_020368
- SwissProt
- Q9NQZ2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MVGRSRRRGA AKWAAVRAKA GPTLTDENGD DLGLPPSPGD TSYYQDQVDD FHEARSRAAL61AKGWNEVQSG DEEDGEEEEE EVLALDMDDE DDEDGGNAGE EEEEENADDD GGSSVQSEAE121ASVDPSLSWG QRKKLYYDTD YGSKSRGRQS QQEAEEEERE EEEEAQIIQR RLAQALQEDD181FGVAWVEAFA KPVPQVDEAE TRVVKDLAKV SVKEKLKMLR KESPELLELI EDLKVKLTEV241KDELEPLLEL VEQGIIPPGK GSQYLRTKYN LYLNYCSNIS FYLILKARRV PAHGHPVIER301LVTYRNLINK LSVVDQKLSS EIRHLLTLKD DAVKKELIPK AKSTKPKPKS VSKTSAAACA361VTDLSDDSDF DEKAKLKYYK EIEDRQKLKR KKEENSTEEQ ALEDQNAKRA ITYQIAKNRG421LTPRRKKIDR NPRVKHREKF RRAKIRRRGQ VREVRKEEQR YSGELSGIRA GVKKSIKLKT481RTRPLEQKLI SEEDLAANDI LDYKDDDDKV
510 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.