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USPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USPL1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USPL1 antibodies →
$504.00each
Catalog Number: LS-G100645-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USPL1 (NM_005800), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA121O19.1 | C13orf22 | D13S106 | D13S106E | Highly charged protein | RP11-121O19.1 | USPL1
Origin Species
Human
NCBI GenBank
NM_005800
SwissProt
Q5W0Q7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
120.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMDSPKIGNG LPVIGPGTDI GISSLHMVGY LGKNFDSAKV PSDEYCPACR EKGKLKALKT
61YRISFQESIF LCEDLQCIYP LGSKSLNNLI SPDLEECHTP HKPQKRKSLE SSYKDSLLLA
121NSKKTRNYIA IDGGKVLNSK HNGEVYDETS SNLPDSSGQQ NPIRTADSLE RNEILEADTV
181DMATTKDPAT VDVSGTGRPS PQNEGCTSKL EMPLESKCTS FPQALCVQWK NAYALCWLDC
241ILSALVHSEE LKNTVTGLCS KEESIFWRLL TKYNQANTLL YTSQLSGVKD GDCKKLTSEI
301FAEIETCLNE VRDEIFISLQ PQLRCTLGDM ESPVFAFPLL LKLETHIEKL FLYSFSWDFE
361CSQCGHQYQN RHMKSLVTFT NVIPEWHPLN AAHFGPCNNC NSKSQIRKMV LEKVSPIFML
421HFVEGLPQND LQHYAFHFEG CLYQITSVIQ YRANNHFITW ILDADGSWLE CDDLKGPCSE
481RHKKFEVPAS EIHIVIWERK ISQVTDKEAA CLPLKKTNDQ HALSNEKPVS STSCSVGDAA
541SAETASVTHP KDISVAPRTL SQDTAVTHGD HLLSGPKGLV DNILPLTLEE TIQKTASVSQ
601LNSEAFLLEN KPVAENTGIL KTNTLLSQES LMASSVSAPC NEKLIQDQFV DISFPSQVVN
661TNMQSVQLNT EDTVNTKSVN NTDATGLIQG VKSVEIEKDA QLKQFLTPKT EQLKPERVTS
721QVSNLKKKET TADSQTTTSK SLQNQSLKEN QKKPFVGSWV KGLISRGASF MPLCVSAHNR
781NTITDLQPSV KGVNNFGGFK TKGINQKASH VSKKARKSAS KPPPISKPPA GPPSSNGTAA
841HPHAHAASEV LEKSGSTSCG AQLNHSSYGN GISSANHEDL VEGQIHKLRL KLRKKLKAEK
901KKLAALMSSP QSRTVRSENL EQVPQDGSPN DCESIEDLLN ELPYPIDIAN ESACTTVPGV
961SLYSSQTHEE ILAELLSPTP VSTELSENGE GDFRYLGMGD SHIPPPVPSE FNDVSQNTHL
1021RQDHNYCSPT KKNPCEVQPD SLTNNACVRT LNLESPMKTD IFDEFFSSSA LNALANDTLD
1081LPHFDEYLFE NY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1092 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.