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USP6NL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP6NL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USP6NL antibodies →
$725.00each
Catalog Number: LS-G119650-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP6NL (NM_001080491), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0019 | RN-tre | RNTRE | TRE2NL | USP6 N-terminal like | USP6NL | USP6 N-terminal-like protein
Origin Species
Human
NCBI GenBank
D13644
SwissProt
Q92738

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRTEAVVNSQ GQTDYLTKDS DQDVALKLAQ ERAEIVAKYD RGREGAEIEP WEDADYLVYK
61VTDRFGFLHE EELPDHNVAV ERQKHLEIER TTKWLKMLKG WEKYKNTEKF HRRIYKGIPL
121QLRGEVWALL LEIPKMKEET RDLYSKLKHR ARGCSPDIRQ IDLDVNRTFR DHIMFRDRYG
181VKQQSLFHVL AAYSIYNTEV GYCQGMSQIT ALLLMYMNEE DAFWALVKLF SGPKHAMHGF
241FVQGFPKLLR FQEHHEKILN KFLSKLKQHL DSQEIYTSFY TMKWFFQCFL DRTPFTLNLR
301IWDIYIFEGE RVLTAMSYTI LKLHKKHLMK LSMEELVEFF QETLAKDFFF EDDFVIEQLQ
361ISMTELKRAK LDLPEPGKED EYPKKPLGQL PPELQSWGVH HLSNGQRSVG RPSPLASGRR
421ESGAPHRRHE HSPHPQSRTG TPERAQPPRR KSVEEESKKL KDEADFQRKL PSGPQDSSRQ
481YNHAAANQNS NATSNIRKEF VPKWNKPSDV SATERTAKYT MEGKGRAAHP ALAVTVPGPA
541EVRVSNVRPK MKALDAEDGK RGSTASQYDN VPGPELDSGA SVEEALERAY SQSPRHALYP
601PSPRKHAEPS SSPSKVSNKF TFKVQPPSHA RYPSQLDGEA RGLAHPPSYS NPPVYHGNSP
661KHFPTANSSF ASPQFSPGTQ LNPSRRPHGS TLSVSASPEK SYSRPSPLVL PSSRIEVLPV
721DTGAGGYSGN SGSPKNGKLI IPPVDYLPDN RTWSEVSYTY RPETQGQSWT RDASRGNLPK
781YSAFQLAPFQ DHGLPAVSVD SPVRYKASPA AEDASPSGYP YSGPPPPAYH YRNRDGLSIQ
841ESVLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

845 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.