LSBio
USP53 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP53 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USP53 antibodies →
$725.00each
Catalog Number: LS-G111469-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP53 (NM_019050), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1350 | USP53 | Ubiquitin specific protease 53
Origin Species
Human
SwissProt
Q70EK8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
120.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAWVKFLRKP GGNLGKVYQP GSMLSLAPTK GLLNEPGQNS CFLNSAVQVL WQLDIFRRSL
61RVLTGHVCQG DACIFCALKT IFAQFQHSRE KALPSDNIRH ALAESFKDEQ RFQLGLMDDA
121AECFENMLER IHFHIVPSRD ADMCTSKSCI THQKFAMTLY EQCVCRSCGA SSDPLPFTEF
181VRYISTTALC NEVERMLERH ERFKPEMFAE LLQAANTTDD YRKCPSNCGQ KIKIRRVLMN
241CPEIVTIGLV WDSEHSDLTE AVVRNLATHL YLPGLFYRVT DENAKNSELN LVGMICYTSQ
301HYCAFAFHTK SSKWVFFDDA NVKEIGTRWK DVVSKCIRCH FQPLLLFYAN PDGTAVSTED
361ALRQVISWSH YKSVAENMGC EKPVIHKSDN LKENGFGDQA KQRENQKFPT DNISSSNRSH
421SHTGVGKGPA KLSHIDQREK IKDISRECAL KAIEQKNLLS SQRKDLEKGQ RKDLGRHRDL
481VDEDLSHFQS GSPPAPNGFK QHGNPHLYHS QGKGSYKHDR VVPQSRASAQ IISSSKSQIL
541APGEKITGKV KSDNGTGYDT DSSQDSRDRG NSCDSSSKSR NRGWKPMRET LNVDSIFSES
601EKRQHSPRHK PNISNKPKSS KDPSFSNWPK ENPKQKGLMT IYEDEMKQEI GSRSSLESNG
661KGAEKNKGLV EGKVHGDNWQ MQRTESGYES SDHISNGSTN LDSPVIDGNG TVMDISGVKE
721TVCFSDQITT SNLNKERGDC TSLQSQHHLE GFRKELRNLE AGYKSHEFHP ESHLQIKNHL
781IKRSHVHEDN GKLFPSSSLQ IPKDHNAREH IHQSDEQKLE KPNECKFSEW LNIENSERTG
841LPFHVDNSAS GKRVNSNEPS SLWSSHLRTV GLKPETAPLI QQQNIMDQCY FENSLSTECI
901IRSASRSDGC QMPKLFCQNL PPPLPPKKYA ITSVPQSEKS ESTPDVKLTE VFKATSHLPK
961HSLSTASEPS LEVSTHMNDE RHKETFQVRE CFGNTPNCPS SSSTNDFQAN SGAIDAFCQP
1021ELDSISTCPN ETVSLTTYFS VDSCMTDTYR LKYHQRPKLS FPESSGFCNN SLS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1073 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.