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USP51 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP51 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USP51 antibodies →
$725.00each
Catalog Number: LS-G103291-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP51 (NM_201286), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_201286

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQVRETSLP SGSGVRWISG GGGGASPEEA VEKAGKMEEA AAGATKASSR REAEEMKLEP
61LQEREPAPEE NLTWSSSGGD EKVLPSIPLR CHSSSSPVCP RRKPRPRPQP RARSRSQPGL
121SAPPPPPARP PPPPPPPPPP APRPRAWRGS RRRSRPGSRP QTRRSCSGDL DGSGDPGGLG
181DWLLEVEFGQ GPTGCSHVES FKVGKNWQKN LRLIYQRFVW SGTPETRKRK AKSCICHVCS
241THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI
301AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEPKLVKPKK KRRKKSVYTV
361GLRGLINLGN TCFMNCIVQA LTHIPLLKDF FLSDKHKCIM TSPSLCLVCE MSSLFHAMYS
421GSRTPHIPYK LLHLIWIHAE HLAGYRQQDA HEFLIAILDV LHRHSKDDSG GQEANNPNCC
481NCIIDQIFTG GLQSDVTCQA CHSVSTTIDP CWDISLDLPG SCATFDSQNP ERADSTVSRD
541DHIPGIPSLT DCLQWFTRPE HLGSSAKIKC NSCQSYQEST KQLTMKKLPI VACFHLKRFE
601HVGKQRRKIN TFISFPLELD MTPFLASTKE SRMKEGQPPT DCVPNENKYS LFAVINHHGT
661LESGHYTSFI RQQKDQWFSC DDAIITKATI EDLLYSEGYL LFYHKQGLEK D
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

711 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.