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USP49 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP49 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USP49 antibodies →
$504.00each
Catalog Number: LS-G111678-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP49 (NM_018561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 49 | Ubiquitin specific protease 49 | Ubiquitin thiolesterase 49 | Ubiquitin thioesterase 49 | USP49
Origin Species
Human
NCBI GenBank
NM_018561
SwissProt
Q70CQ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
73.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDRCKHVGRL RLAQDHSILN PQKWCCLECA TTESVWACLK CSHVACGRYI EDHALKHFEE
61TGHPLAMEVR DLYVFCYLCK DYVLNDNPEG DLKLLRSSLL AVRGQKQDTP VRRGRTLRSM
121ASGEDVVLPQ RAPQGQPQML TALWYRRQRL LARTLRLWFE KSSRGQAKLE QRRQEEALER
181KKEEARRRRR EVKRRLLEEL ASTPPRKSAR LLLHTPRDAG PAASRPAALP TSRRVPAATL
241KLRRQPAMAP GVTGLRNLGN TCYMNSILQV LSHLQKFREC FLNLDPSKTE HLFPKATNGK
301TQLSGKPTNS SATELSLRND RAEACEREGF CWNGRASISR SLELIQNKEP SSKHISLCRE
361LHTLFRVMWS GKWALVSPFA MLHSVWSLIP AFRGYDQQDA QEFLCELLHK VQQELESEGT
421TRRILIPFSQ RKLTKQVLKV VNTIFHGQLL SQVTCISCNY KSNTIEPFWD LSLEFPERYH
481CIEKGFVPLN QTECLLTEML AKFTETEALE GRIYACDQCN SKRRKSNPKP LVLSEARKQL
541MIYRLPQVLR LHLKRFRWSG RNHREKIGVH VVFDQVLTME PYCCRDMLSS LDKETFAYDL
601SAVVMHHGKG FGSGHYTAYC YNTEGGACAL LCGVGDTERG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

640 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.