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USP47 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP47 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USP47 antibodies →
$725.00each
Catalog Number: LS-G112103-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP47 (NM_017944), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 47 | Ubiquitin thiolesterase 47 | Ubiquitin specific protease 47 | Ubiquitin thioesterase 47 | USP47 | TRFP
Origin Species
Human
NCBI GenBank
NM_017944
SwissProt
Q96K76

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
147 kDa
Protein Type
Lysate
Amino Acid Sequence
1MVPGEENQLV PKEAPLDHTS DKSLLDANFE PGKKNFLHLT DKDGEQPQIL LEDSSAGEDS
61VHDRFIGPLP REGSGGSTSD YVSQSYSYSS ILNKSETGYV GLVNQAMTCY LNSLLQTLFM
121TPEFRNALYK WEFEESEEDP VTSIPYQLQR LFVLLQTSKK RAIETTDVTR SFGWDSSEAW
181QQHDVQELCR VMFDALEQKW KQTEQADLIN ELYQGKLKDY VRCLECGYEG WRIDTYLDIP
241LVIRPYGSSQ AFASVEEALH AFIQPEILDG PNQYFCERCK KKCDARKGLR FLHFPYLLTL
301QLKRFDFDYT TMHRIKLNDR MTFPEELDMS TFIDVEDEKS PQTESCTDSG AENEGSCHSD
361QMSNDFSNDD GVDEGICLET NSGTEKISKS GLEKNSLIYE LFSVMVHSGS AAGGHYYACI
421KSFSDEQWYS FNDQHVSRIT QEDIKKTHGG SSGSRGYYSS AFASSTNAYM LIYRLKDPAR
481NAKFLEVDEY PEHIKNLVQK ERELEEQEKR QREIERNTCK IKLFCLHPTK QVMMENKLEV
541HKDKTLKEAV EMAYKMMDLE EVIPLDCCRL VKYDEFHDYL ERSYEGEEDT PMGLLLGGVK
601STYMFDLLLE TRKPDQVFQS YKPGEVMVKV HVVDLKAESV AAPITVRAYL NQTVTEFKQL
661ISKAIHLPAE TMRIVLERCY NDLRLLSVSS KTLKAEGFFR SNKVFVESSE TLDYQMAFAD
721SHLWKLLDRH ANTIRLFVLL PEQSPVSYSK RTAYQKAGGD SGNVDDDCER VKGPVGSLKS
781VEAILEESTE KLKSLSLQQQ QDGDNGDSSK STETSDFENI ESPLNERDSS ASVDNRELEQ
841HIQTSDPENF QSEERSDSDV NNDRSTSSVD SDILSSSHSS DTLCNADNAQ IPLANGLDSH
901SITSSRRTKA NEGKKETWDT AEEDSGTDSE YDESGKSRGE MQYMYFKAEP YAADEGSGEG
961HKWLMVHVDK RITLAAFKQH LEPFVGVLSS HFKVFRVYAS NQEFESVRLN ETLSSFSDDN
1021KITIRLGRAL KKGEYRVKVY QLLVNEQEPC KFLLDAVFAK GMTVRQSKEE LIPQLREQCG
1081LELSIDRFRL RKKTWKNPGT VFLDYHIYEE DINISSNWEV FLEVLDGVEK MKSMSQLAVL
1141SRRWKPSEMK LDPFQEVVLE SSSVDELREK LSEISGIPLD DIEFAKGRGT FPCDISVLDI
1201HQDLDWNPKV STLNVWPLYI CDDGAVIFYR DKTEELMELT DEQRNELMKK ESSRLQKTGH
1261RVTYSPRKEK ALKIYLDGAP NKDLTQD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1287 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.