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USP40 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP40 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all USP40 antibodies →
$725.00each
Catalog Number: LS-G111876-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP40 (NM_018218), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 40 | Ubiquitin specific protease 40 | Ubiquitin thioesterase 40 | Ubiquitin thiolesterase 40 | USP40
Origin Species
Human
NCBI GenBank
AK001647
SwissProt
Q9NVE5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
141.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLFLRVVFS FTMFGDLFEE EYSTVSNNQY GKGKKLKTKA LEPPAPREFT NLSGIRNQGG
61TCYLNSLLQT LHFTPEFREA LFSLGPEELG LFEDKDKPDA KVRIIPLQLQ RLFAQLLLLD
121QEAASTADLT DSFGWTSNEE MRQHDVQELN RILFSALETS LVGTSGHDLI YRLYHGTIVN
181QIVCKECKNV SERQEDFLDL TVAVKNVSGL EDALWNMYVE EEVFDCDNLY HCGTCDRLVK
241AAKSAKLRKL PPFLTVSLLR FNFDFVKCER YKETSCYTFP LRINLKPFCE QSELDDLEYI
301YDLFSVIIHK GGCYGGHYHV YIKDVDHLGN WQFQEEKSKP DVNLKDLQSE EEIDHPLMIL
361KAILLEENNL IPVDQLGQKL LKKIGISWNK KYRKQHGPLR KFLQLHSQIF LLSSDESTVR
421LLKNSSLQAE SDFQRNDQQI FKMLPPESPG LNNSISCPHW FDINDSKVQP IREKDIEQQF
481QGKESAYMLF YRKSQLQRPP EARANPRYGV PCHLLNEMDA ANIELQTKRA ECDSANNTFE
541LHLHLGPQYH FFNGALHPVV SQTESVWDLT FDKRKTLGDL RQSIFQLLEF WEGDMVLSVA
601KLVPAGLHIY QSLGGDELTL CETEIADGED IFVWNGVEVG GVHIQTGIDC EPLLLNVLHL
661DTSSDGEKCC QVIESPHVFP ANAEVGTVLT ALAIPAGVIF INSAGCPGGE GWTAIPKEDM
721RKTFREQGLR NGSSILIQDS HDDNSLLTKE EKWVTSMNEI DWLHVKNLCQ LESEEKQVKI
781SATVNTMVFD IRIKAIKELK LMKELADNSC LRPIDRNGKL LCPVPDSYTL KEAELKMGSS
841LGLCLGKAPS SSQLFLFFAM GSDVQPGTEM EIVVEETISV RDCLKLMLKK SGLQGDAWHL
901RKMDWCYEAG EPLCEEDATL KELLICSGDT LLLIEGQLPP LGFLKVPIWW YQLQGPSGHW
961ESHQDQTNCT SSWGRVWRAT SSQGASGNEP AQVSLLYLGD IEISEDATLA ELKSQAMTLP
1021PFLEFGVPSP AHLRAWTVER KRPGRLLRTD RQPLREYKLG RRIEICLEPL QKGENLGPQD
1081VLLRTQVRIP GERTYAPALD LVWNAAQGGT AGSLRQRVAD FYRLPVEKIE IAKYFPEKFE
1141WLPISSWNQQ ITKRKKKKKQ DYLQGAPYYL KDGDTIGVKN LLIDDDDDFS TIRDDTGKEK
1201QKQRALGRRK SQEALHEQSS YILSSAETPA RPRAPETSLS IHVGSFR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1247 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.