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USP16 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

USP16 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120876-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human USP16 (NM_001032410), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Deubiquitinating enzyme 16 | Ubiquitin thiolesterase 16 | UBP-M | USP16 | Ubiquitin specific protease 16 | Ubiquitin thioesterase 16
Origin Species
Human
NCBI GenBank
NM_006447
SwissProt
Q9Y5T5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
93.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MGKKRTKGKT VPIDDSSETL EPVCRHIRKG LEQGNLKKAL VNVEWNICQD CKTDNKVKDK
61AEEETEEKPS VWLCLKCGHQ GCGRNSQEQH ALKHYLTPRS EPHCLVLSLD NWSVWCYVCD
121NEVQYCSSNQ LGQVVDYVRK HASITTPKPE KDNGNIELEN KKLEKESKNE QEREKKENMA
181KENPPMNSPC QITVKGLSNL GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL
241ALTEPLEINL EPPGPLTLAM SQFLNEMQET KKGVVTPKEL FSQVCKKAVR FKGYQQQDSQ
301ELLRYLLDGM RAEEHQRVSK GILKAFGNST EKLDEELKNK VKDYEKKKSM PSFVDRIFGG
361ELTSMIMCDQ CRTVSLVHES FLDLSLPVLD DQSGKKSVND KNLKKTVEDE DQDSEEEKDN
421DSYIKERSDI PSGTSKHLQK KAKKQAKKQA KNQRRQQKIQ GKVLHLNDIC TIDHPEDSDN
481EAEMSLQGEV NIKSNHISQE GVMHKEYCVN QKDLNGQAKM IESVTDNQKS TEEVDMKNIN
541MDNDLEVLTS SPTRNLNGAY LTEGSNGEVD ISNGFKNLNL NAALHPDEIN IEILNDSHTP
601GTKVYEVVNE DPETAFCTLA NREVFNTDEC SIQHCLYQFT RNEKLRDANK LLCEVCTRRQ
661CNGPKANIKG ERKHVYTNAK KQMLISLAPP VLTLHLKRFQ QAGFNLRKVN KHIKFPEILD
721LAPFCTLKCK NVAEENTRVL YSLYGVVEHS GTMRSGHYTA YAKARTANSH LSNLVLHGDI
781PQDFEMESKG QWFHISDTHV QAVPTTKVLN SQAYLLFYER IL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

822 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.