Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
URP2 / FERMT3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102007-100
Catalog Number: LS-G102007
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human URP2(FERMT3) (NM_031471), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Fermitin family member 3 | FERMT3 | Fermitin family homolog 3 | Kindlin-3 | KIND3 | Mig-2 | MIG2B | UNC-112 related protein 2 | Unc-112-related protein 2 | UNC112C | Kindlin 3 | MIG2-like protein | URP2 | URP2SF
- Origin Species
- Human
- NCBI GenBank
- NM_031471
- SwissProt
- Q86UX7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGMKTASGD YIDSSWELRV FVGEEDPEAE SVTLRVTGES HIGGVLLKIV EQINRKQDWS61DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV ILRLPNRRAL RLRASFSQPL121FQAVAAICRL LSIRHPEELS LLRAPEKKEK KKKEKEPEEE LYDLSKVVLA GGVAPALFRG181MPAHFSDSAQ TEACYHMLSR PQPPPDPLLL QRLPRPSSLS DKTQLHSRWL DSSRCLMQQG241IKAGDALWLR FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL LEEIDCTEEE MMVFAALQYH301INKLSQSGEV GEPAGTDPGL DDLDVALSNL EVKLEGSAPT DVLDSLTTIP ELKDHLRIFR361PRKLTLKGYR QHWVVFKETT LSYYKSQDEA PGDPIQQLNL KGCEVVPDVN VSGQKFCIKL421LVPSPEGMSE IYLRCQDEQQ YARWMAGCRL ASKGRTMADS SYTSEVQAIL AFLSLQRTGS481GGPGNHPHGP DASAEGLNPY GLVAPRFQRK FKAKQLTPRI LEAHQNVAQL SLAEAQLRFI541QAWQSLPDFG ISYVMVRFKG SRKDEILGIA NNRLIRIDLA VGDVVKTWRF SNMRQWNVNW601DIRQVAIEFD EHINVAFSCV SASCRIVHEY IGGYIFLSTR ERARGEELDE DLFLQLTGGH661EAFTRTRPLE QKLISEEDLA ANDILDYKDD DDKV
694 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.