Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
URG4 / URGCP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120044-100
Catalog Number: LS-G120044
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human URG4(URGCP) (NM_001077663), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1507 | Up-regulated gene 4 | URGCP | URG4
- Origin Species
- Human
- NCBI GenBank
- NM_017920
- SwissProt
- Q8TCY9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 104.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASPGIEVEL LGKGHSDLGE VAPEIKASER RTAVAIADLE WREMEGDDCE FRYGDGTNEA61QDNDFPTVER SRLQEMLSLL GLETYQVQKL SLQDSLQISF DSMKNWAPQV PKDLPWNFLR121KLQALNADAR NTTMVLDVLP DARPVEKESQ MEEEIIYWDP ADDLAADIYS FSELPTPDTP181VNPLDLLCAL LLSSDSFLQQ EIALKMALCQ FALPLVLPDS ENHYHTFLLW AMRGIVRTWW241SQPPRGMGSF REDSVVLSRA PAFAFVRMDV SSNSKSQLLN AVLSPGHRQW DCFWHRDLNL301GTNAREISDG LVEISWFFPS GREDLDIFPE PVAFLNLRGD IGSHWLQFKL LTEISSAVFI361LTDNISKKEY KLLYSMKEST TKYYFILSPY RGKRNTNLRF LNKLIPVLKI DHSHVLVKVS421STDSDSFVKR IRAIVGNVLR APCRRVSVED MAHAARKLGL KVDEDCEECQ KAKDRMERIT481RKIKDSDAYR RDELRLQGDP WRKAAQVEKE FCQLQWAVDP PEKHRAELRR RLLELRMQQN541GHDPSSGVQE FISGISSPSL SEKQYFLRWM EWGLARVAQP RLRQPPETLL TLRPKHGGTT601DVGEPLWPEP LGVEHFLREM GQFYEAESCL VEAGRLPAGQ RRFAHFPGLA SELLLTGLPL661ELIDGSTLSM PVRWVTGLLK ELHVRLERRS RLVVLSTVGV PGTGKSTLLN TMFGLRFATG721KSCGPRGAFM QLITVAEGFS QDLGCDHILV IDSGGLIGGA LTSAGDRFEL EASLATLLMG781LSNVTVISLA ETKDIPAAIL HAFLRLEKTG HMPNYQFVYQ NLHDVSVPGP RPRDKRQLLD841PPGDLSRAAA QMEKQGDGFR ALAGLAFCDP EKQHIWHIPG LWHGAPPMAA VSLAYSEAIF901ELKRCLLENI RNGLSNQNKN IQQLIELVRR LTRTRPLEQKLISEEDLAANDILDYKDDDDKV
931 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.