Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
UPK1A / Uroplakin 1A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114931-100
Catalog Number: LS-G114931
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Uroplakin Ia(UPK1A) (NM_007000), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- UP1A | UPIA | UPK1A | Uroplakin-1a | Tetraspanin-21 | Tspan-21 | TSPAN21 | UPKA | Uroplakin 1A | Uroplakin Ia
- Origin Species
- Human
- NCBI GenBank
- NM_007000
- SwissProt
- O00322
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 28.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASAAAAEAE KGSPVVVGLL VVGNIIILLS GLSLFAETIW VTADQYRVYP LMGVSGKDDV61FAGAWIAIFC GFSFFMVASF GVGAALCRRR SMVLTYLVLM LIVYIFECAS CITSYTHRDY121MVSNPSLITK QMLTFYSADT DQGQELTRLW DRVMIEQECC GTSGPMDWVN FTSAFRAATP181EVVFPWPPLC CRRTGNFIPL NEEGCRLGHM DYLFTKGCFE HIGHAIDSYT WGISWFGFAI241LMWTLPVMLI AMYFYTMLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
258 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.