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UNKL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNKL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UNKL antibodies →
$725.00each
Catalog Number: LS-G131088-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RING finger protein unkempt like(UNKL) (NM_001193388), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C16orf28 | IGF-like family member 1 | ZC3H5L | Unkempt-like | UNKL | ZC3HDC5L
Origin Species
Human
NCBI GenBank
NM_023076
SwissProt
Q9H9P5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSVSKAAAA ALSGSPPQTE KPTHYRYLKE FRTEQCPLFS QHKCAQHRPF TCFHWHFLNQ
61RRRRPLRRRD GTFNYSPDVY CSKYNEATGV CPDGDECPYL HRTTGDTERK YHLRYYKTGT
121CIHETDARGH CVKNGLHCAF AHGPLDLRPP VCDVRELQAQ EALQNGQLGG GEGVPDLQPG
181VLASQAMIEK ILSEDPRWQD ANFVLGSYKT EQCPKPPRLC RQGYACPHYH NSRDRRRNPR
241RFQYRSTPCP SVKHGDEWGE PSRCDGGDGC QYCHSRTEQQ FHPESTKCND MRQTGYCPRG
301PFCAFAHVEK SLGMVNEWGC HDLHLTSPSS TGSGQPGNAK RRDSPAEGGP RGSEQDSKQN
361HLAVFAAVHP PAPSVSSSVA SSLASSAGSG SSSPTALPAP PARALPLGPA SSTVEAVLGS
421ALDLHLSNVN IASLEKDLEE QDGHDLGAAG PRSLAGSAPV AIPGSLPRAP SLHSPSSAST
481SPLGSLSQPL PGPVGSSAMT PPQQPPPLRS EPGTLGSAAS SYSPLGLNGV PGSIWDFVSG
541SFSPSPSPIL SAGPPSSSSA SPNGAELARV RRQLDEAKRK IRQWEESWQQ VKQVCDAWQR
601EAQEAKERAR VADSDRQLAL QKKEEVEAQV IFQLRAKQCV ACRERAHGAV LRPCQHHILC
661EPCAATAPEC PYCKGQPLQW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

680 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.