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UNK Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNK Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G120205-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UNK unkempt(UNK) (NM_001080419), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1753 | ZC3H5 | Unkempt homolog (Drosophila) | UNK | ZC3HDC5
Origin Species
Human
NCBI GenBank
AI290504

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRPDCNSQKP SQALSIRDTS SKDPWPDIEP QLPATPAAVW GVFTFSWRGA GALGPRRGPR
61RLNNKRGAAK RQEDKTMSKG PGPGGSAASS APPAATAQVL QAQPEKPQHY TYLKEFRTEQ
121CPLFVQHKCT QHRPYTCFHW HFVNQRRRRS IRRRDGTFNY SPDVYCTKYD EATGLCPEGD
181ECPFLHRTTG DTERRYHLRY YKTGICIHET DSKGNCTKNG LHCAFAHGPH DLRSPVYDIR
241ELQAMEALQN GQTTVEGSIE GQSAGAASHA MIEKILSEEP RWQETAYVLG NYKTEPCKKP
301PRLCRQGYAC PYYHNSKDRR RSPRKHKYRS SPCPNVKHGD EWGDPGKCEN GDACQYCHTR
361TEQQFHPEIY KSTKCNDMQQ SGSCPRGPFC AFAHVEQPPL SDDLQPSSAV SSPTQPGPVL
421YMPSAAGDSV PVSPSSPHAP DLSALLCRNS SLGSPSNLCG SPPGSIRKPP NLEGIVFPGE
481SGLAPGSYKK APGFEREDQV GAEYLKNFKC QAKLKPHSLE PRSQEQPLLQ PKQDMLGILP
541AGSPLTSSIS SSITSSLAAT PPSPVGTSSV PGMNANALPF YPTSDTVESV IESALDDLDL
601NEFGVAALEK TFDNSTVPHP GSITIGGSLL QSSAPVNIPG SLGSSASFHS ASPSPPVSLS
661SHFLQQPQGH LSQSENTFLG TSASHGSLGL NGMNSSIWEH FASGSFSPGT SPAFLSGPGA
721AELARLRQEL DEANSTIKQW EESWKQAKQA CDAWKKEAEE AGERASAAGA ECELAREQRD
781ALEVQVKKLQ EELERLHAGP EPQALPAFSD LEALSLSTLY SLQKQLRAHL EQVDKAVFHM
841QSVKCLKCQE QKRAVLPCQH AALCELCAEG SECPICQPGR AHTLQS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

886 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.