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UNC5CL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNC5CL Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UNC5CL antibodies →
$504.00each
Catalog Number: LS-G105208-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ZU5(UNC5CL) (NM_173561), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
MUXA | ZUD | UNC5C-like protein | Protein unc-5 homolog C-like | UNC5CL
Origin Species
Human
NCBI GenBank
NM_173561
SwissProt
Q8IV45

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCPQESSFQP SQFLLLVGVP VASVLLLAQC LRWHCPRRLL GACWTLNGQE EPVSQPTPQL
61ENEVSRQHLP ATLPEMVAFY QELHTPTQGQ TMVRQLMHKL LVFSAREVDH RGGCLMLQDT
121GISLLIPPGA VAVGRQERVS LILVWDLSDA PSLSQAQGLV SPVVACGPHG ASFLKPCTLT
181FKHCAEQPSH ARTYSSNTTL LDAKVWRPLG RPGAHASRDE CRIHLSHFSL YTCVLEAPVG
241REARKWLQLA VFCSPLVPGQ SHLQLRIYFL NNTPCALQWA LTNEQPHGGR LRGPCQLFDF
301NGARGDQCLK LTYISEGWEN VDDSSCQLVP HLHIWHGKCP FRSFCFRRKA ADENEDCSAL
361TNEIIVTMHT FQDGLETKYM EILRFQASEE ESWAAPPPVS QPPPCNRLPP ELFEQLRMLL
421EPNSITGNDW RGLASHLGLC GMKIRFLSCQ RSPAAAILEL FEEQNGSLQE LHYLMTVMER
481LDCASAIQNY LSGTHGGSPG PERGGARDNQ GLELDEKL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.