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UNC5A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNC5A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UNC5A antibodies →
$725.00each
Catalog Number: LS-G107539-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UNC5A (NM_133369), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1976 | Netrin receptor Unc5h1 | Netrin receptor UNC5A | Unc-5 homolog 1 | Unc-5 homolog A (C. elegans) | UNC5H1 | Protein unc-5 homolog 1 | Protein unc-5 homolog A | Unc5 (C.elegans homolog) a | UNC5A
Origin Species
Human
NCBI GenBank
BC009333
SwissProt
Q6ZN44

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
92.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVRPGLWPA LLGIVLAAWL RGSGAQQSAT VANPVPGANP DLLPHFLVEP EDVYIVKNKP
61VLLVCKAVPA TQIFFKCNGE WVRQVDHVIE RSTDGSSGLP TMEVRINVSR QQVEKVFGLE
121EYWCQCVAWS SSGTTKSQKA YIRIAYLRKN FEQEPLAKEV SLEQGIVLPC RPPEGIPPAE
181VEWLRNEDLV DPSLDPNVYI TREHSLVVRQ ARLADTANYT CVAKNIVARR RSASAAVIVY
241VDGSWSPWSK WSACGLDCTH WRSRECSDPA PRNGGEECQG TDLDTRNCTS DLCVHTASGP
301EDVALYVGLI AVAVCLVLLL LVLILVYCRK KEGLDSDVAD SSILTSGFQP VSIKPSKADN
361PHLLTIQPDL STTTTTYQGS LCPRQDGPSP KFQLTNGHLL SPLGGGRHTL HHSSPTSEAE
421EFVSRLSTQN YFRSLPRGTS NMTYGTFNFL GGRLMIPNTG ISLLIPPDAI PRGKIYEIYL
481TLHKPEDVRL PLAGCQTLLS PIVSCGPPGV LLTRPVILAM DHCGEPSPDS WSLRLKKQSC
541EGSWEDVLHL GEEAPSHLYY CQLEASACYV FTEQLGRFAL VGEALSVAAA KRLKLLLFAP
601VACTSLEYNI RVYCLHDTHD ALKEVVQLEK QLGGQLIQEP RVLHFKDSYH NLRLSIHDVP
661SSLWKSKLLV SYQEIPFYHI WNGTQRYLHC TFTLERVSPS TSDLACKLWV WQVEGDGQSF
721SINFNITKDT RFAELLALES EAGVPALVGP SAFKIPFLIR QKIISSLDPP CRRGADWRTL
781AQKLHLDSHL SFFASKPSPT AMILNLWEAR HFPNGNLSQL AAAVAGLGQP DAGLFTVSEA
841EC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

842 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.