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UNC45B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNC45B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UNC45B antibodies →
$725.00each
Catalog Number: LS-G102430-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UNC45B (NM_173167), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CMYA4 | Protein unc-45 homolog B | Unc-45 homolog B (C. elegans) | UNC45 | UNC45B | SMUNC45 | UNC-45B | Cardiomyopathy associated 4 | Striated muscle UNC45
Origin Species
Human
SwissProt
Q8IWX7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAEVEAVQLK EEGNRHFQLQ DYKAATNSYS QALKLTKDKA LLATLYRNRA ACGLKTESYI
61QAASDASRAI DINSSDIKAL YRRCQALEHL GKLDQAFKDV QRCATLEPRN QNFQEMLRRL
121NTSIQEKLRV QFSTDSRVQK MFEILLDENS EADKREKAAN NLIVLGREEA GAEKIFQNNG
181VALLLQLLDT KKPELVLAAV RTLSGMCSGH QARATVILHA VRIDRICSLM AVENEEMSLA
241VCNLLQAIID SLSGEDKREH RGKEEALVLD TKKDLKQITS HLLDMLVSKK VSGQGRDQAL
301NLLNKNVPRK DLAIHDNSRT IYVVDNGLRK ILKVVGQVPD LPSCLPLTDN TRMLASILIN
361KLYDDLRCDP ERDHFRKICE EYITGKFDPQ DMDKNLNAIQ TVSGILQGPF DLGNQLLGLK
421GVMEMMVALC GSERETDQLV AVEALIHAST KLSRATFIIT NGVSLLKQIY KTTKNEKIKI
481RTLVGLCKLG SAGGTDYGLR QFAEGSTEKL AKQCRKWLCN MSIDTRTRRW AVEGLAYLTL
541DADVKDDFVQ DVPALQAMFE LAKXXTSDKT ILYSVATTLV NCTNSYDVKE VIPELVQLAK
601FSKQHVPEEH PKDKKDFIDM RVKRLLKAGV ISALACMVKA DSAILTDQTK ELLARVFLAL
661CDNPKDRGTI VAQGGGKALI PLALEGTDVG KVKAAHALAK IAAVSNPDIA FPGERVYEVV
721RPLVRLLDTQ RDGLQNYEAL LGLTNLSGRS DKLRQKIFKE RALPDIENYM FENHDQLRQA
781ATECMCNMVL HKEVQERFLA DGNDRLKLVV LLCGEDDDKV QNAAAGALAM LTAAHKKLCL
841KMTQVTTQWL EILQRLCLHD QLSVQHRGLV IAYNLLAADA ELAKKLVESE LLEILTVVGK
901QEPDEKKAEV VQTARECLIK CMDYGFIKPV S
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

931 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.