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UNC45A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

UNC45A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all UNC45A antibodies →
$504.00each
Catalog Number: LS-G99305-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human UNC45A (NM_001039675), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GCUNC-45 | GC-UNC45 | General cell UNC45 | IRO039700 | Protein unc-45 homolog A | UNC45A | UNC-45A | GCUNC45 | SMAP-1 | Unc-45 homolog A (C. elegans)
NCBI GenBank
NM_018671
SwissProt
Q9H3U1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTASSVEQLR KEGNELFKCG DYGGALAAYT QALGLDATPQ DQAVLHRNRA ACHLKLEDYD
61KAETEASKAI EKDGGDVKAL YRRSQALEKL GRLDQAVLDL QRCVSLEPKN KVFQEALRNI
121GGQIQEKVRY MSSTDAKVEQ MFQILLDPEE KGTEKKQKAS QNLVVLARED AGAEKIFRSN
181GVQLLQRLLD MGETDLMLAA LRTLVGICSE HQSRTVATLS ILGTRRVVSI LGVESQAVSL
241AACHLLQVMF DALKEGVKKG FRGKEGAIIV DPARELKVLI SNLLDLLTEV GVSGQGRDNA
301LTLLIKAVPR KSLKDPNNSL TLWVIDQGLK KILEVGGSLQ DPPGELAVTA NSRMSASILL
361SKLFDDLKCD AERENFHRLC ENYIKSWFEG QGLAGKLRAI QTVSCLLQGP CDAGNRALEL
421SGVMESVIAL CASEQEEEQL VAVEALIHAA GKAKRASFIT ANGVSLLKDL YKCSEKDSIR
481IRALVGLCKL GSAGGTDFSM KQFAEGSTLK LAKQCRKWLC NDQIDAGTRR WAVEGLAYLT
541FDADVKEEFV EDAAALKALF QLSRLEERSV LFAVASALVN CTNSYDYEEP DPKMVELAKY
601AKQHVPEQHP KDKPSFVRAR VKKLLAAGVV SAMVCMVKTE SPVLTSSCRE LLSRVFLALV
661EEVEDRGTVV AQGGGRALIP LALEGTDVGQ TKAAQALAKL TITSNPEMTF PGERIYEVVR
721PLVSLLHLNC SGLQNFEALM ALTNLAGISE RLRQKILKEK AVPMIEGYMF EEHEMIRRAA
781TECMCNLAMS KEVQDLFEAQ GNDRLKLLVL YSGEDDELLQ RAAAGGLAML TSMRPTLCSR
841IPQVTTHWLE ILQALLLSSN QELQHRGAVV VLNMVEASRE IASTLMESEM MEILSVLAKG
901DHSPVTRAAA ACLDKAVEYG LIQPNQDGE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

929 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.