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ULK4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ULK4 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ULK4 antibodies →
$725.00each
Catalog Number: LS-G112144-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ULK4 (NM_017886), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM7C1 | ULK4 | Unc-51-like kinase 4 | REC01035
Origin Species
Human
NCBI GenBank
NM_017886
SwissProt
Q96C45

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
142.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENFILYEEI GRGSKTVVYK GRRKGTINFV AILCTDKCKR PEITNWVRLT REIKHKNIVT
61FHEWYETSNH LWLVVELCTG GSLKTVIAQD ENLPEDVVRE FGIDLISGLH HLHKLGILFC
121DISPRKILLE GPGTLKFSNF CLAKVEGENL EEFFALVAAE EGGGDNGENV LKKSMKSRVK
181GSPVYTAPEV VRGADFSISS DLWSLGCLLY EMFSGKPPFF SESISELTEK ILCEDPLPPI
241PKDSSRPKAS SDFINLLDGL LQRDPQKRLT WTRLLQHSFW KKAFAGADQE SSVEDLSLSR
301NTMECSGPQD SKELLQNSQS RQAKGHKSGQ PLGHSFRLEN PTEFRPKSTL EGQLNESMFL
361LSSRPTPRTS TAVEVSPGED MTHCSPQKTS PLTKITSGHL SQQDLESQMR ELIYTDSDLV
421VTPIIDNPKI MKQPPVKFDA KILHLPTYSV DKLLFLKDQD WNDFLQQVCS QIDSTEKSMG
481ASRAKLNLLC YLCVVAGHQE VATRLLHSPL FQLLIQHLRI APNWDIRAKV AHVIGLLASH
541TAELQENTPV VEAIVLLTEL IRENFRNSKL KQCLLPTLGE LIYLVATQEE KKKNPRECWA
601VPLAAYTVLM RCLREGEERV VNHMAAKIIE NVCTTFSAQS QGFITGEIGP ILWYLFRHST
661ADSLRITAVS ALCRITRHSP TAFQNVIEKV GLNSVINSLA SAICKVQQYM LTLFAAMLSC
721GIHLQRLIQE KGFVSTIIRL LDSPSTCIRA KAFLVLLYIL IYNREMLLLS CQARLVMYIE
781RDSRKTTPGK EQQSGNEYLS KCLDLLICHI VQELPRILGD ILNSLANVSG RKHPSTVQVK
841QLKLCLPLMP VVLHLVTSQV FRPQVVTEEF LFSYGTILSH IKSVDSGETN IDGAIGLTAS
901EEFIKITLSA FEAIIQYPIL LKDYRSTVVD YILPPLVSLV QSQNVEWRLF SLRLLSETTS
961LLVNQEFGDG KEKASVDSDS NLLALIRDVL LPQYEHILLE PDPVPAYALK LLVAMTEHNP
1021TFTRLVEESK LIPLIFEVTL EHQESILGNT MQSVIALLSN LVACKDSNME LLYEQGLVSH
1081ICNLLTETAT LCLDVDNKNN NEMAAPLLFS LLDILHSMLT YTSGIVRLAL QAQKSGSGED
1141PQAAEDLLLL NRPLTDLISL LIPLLPNEDP EIFDVSSKCL SILVQLYGGE NPDSLSPENV
1201EIFAHLLTSK EDPKEQKLLL RILRRMITSN EKHLESLKNA GSLLRALERL APGSGSFADS
1261AVAPLALEIL QAVGH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1275 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.