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ULK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ULK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all ULK2 antibodies →
$725.00each
Catalog Number: LS-G126044-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ULK2 (NM_001142610), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ATG1B | KIAA0623 | Unc-51-like kinase 2 | ULK2 | Unc51.2
Origin Species
Human
NCBI GenBank
NM_014683
SwissProt
Q8IYT8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEVVGDFEYS KRDLVGHGAF AVVFRGRHRQ KTDWEVAIKS INKKNLSKSQ ILLGKEIKIL
61KELQHENIVA LYDVQELPNS VFLVMEYCNG GDLADYLQAK GTLSEDTIRV FLHQIAAAMR
121ILHSKGIIHR DLKPQNILLS YANRRKSSVS GIRIKIADFG FARYLHSNMM AATLCGSPMY
181MAPEVIMSQH YDAKADLWSI GTVIYQCLVG KPPFQANSPQ DLRMFYEKNR SLMPSIPRET
241SPYLANLLLG LLQRNQKDRM DFEAFFSHPF LEQGPVKKSC PVPVPMYSGS VSGSSCGSSP
301SCRFASPPSL PDMQHIQEEN LSSPPLGPPN YLQVSKDSAS TSSKNSSCDT DDFVLVPHNI
361SSDHSCDMPM GTAGRRASNE FLVCGGQCQP TVSPHSETAP IPVPTQIRNY QRIEQNLTST
421ASSGTNVHGS PRSAVVRRSN TSPMGFLRPG SCSPVPADTA QTVGRRLSTG SSRPYSPSPL
481VGTIPEQFSQ CCCGHPQGHD SRSRNSSGSP VPQAQSPQSL LSGARLQSAP TLTDIYQNKQ
541KLRKQHSDPV CPSHTGAGYS YSPQPSRPGS LGTSPTKHLG SSPRSSDWFF KTPLPTIIGS
601PTKTTAPFKI PKTQASSNLL ALVTRHGPAE EQSKDGNEPR ECAHCLLVQG SERQRAEQQS
661KAVFGRSVST GKLSDQQGKT PICRHQGSTD SLNTERPMDI APAGACGGVL APPAGTAASS
721KAVLFTVGSP PHSAAAPTCT HMFLRTRTTS VGPSNSGGSL CAMSGRVCVG SPPGPGFGSS
781PPGAEAAPSL RYVPYGASPP SLEGLITFEA PELPEETLME REHTDTLRHL NVMLMFTECV
841LDLTAMRGGN PELCTSAVSL YQIQESVVVD QISQLSKDWG RVEQLVLYMK AAQLLAASLH
901LAKAQIKSGK LSPSTAVKQV VKNLNERYKF CITMCKKLTE KLNRFFSDKQ RFIDEINSVT
961AEKLIYNCAV EMVQSAALDE MFQQTEDIVY RYHKAALLLE GLSRILQDPA DIENVHKYKC
1021SIERRLSALC HSTATV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1036 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.